J Korean Soc Plast Reconstr Surg.
2008 Sep;35(5):626-629.
Gastroepiploic Artery Preservation with Glycerol as a Training Model for Microvascular Anastomosis
- Affiliations
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- 1Department of Plastic and Reconstructive Surgery, Research Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine, Seoul, Korea. hchang@snu.ac.kr
- 2Department of Pathology, Research Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine, Seoul, Korea.
- 3Department of Surgery, Research Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine, Seoul, Korea.
Abstract
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PURPOSE: We investigate the possibility of vessel preservation with glycerol and evaluate the potential of preserved gastroepiploic artery as a tool for the microsurgical practice.
METHODS
In 5 early gastric cancer patients, IRB(No. C-0603-040-170), we gained gastroepiploic artery specimens(5 segments, about 10cm) after gastrectomy. Each segment was rinsed in a normal saline and subsequently placed in a bottle, containing 50mL anhydrous glycerol (87%). The bottles were refrigerated at 4degrees C, the longest preservation time being 5 months.
RESULTS
At first glance, no fragmentation was detected and the surface of vessel seemed mild sclerotic. In histological examination, vascular structures remained intact though preservation with glycerol led to a mild atrophy of the smooth muscle in the tunica media. Especially, we found out the elastic fibers and endothelial lining of the intima were preserved until 5 months. Adequate status for using microsurgical practice was also maintained and leakage test was performed successfully with gentian violet ink.
CONCLUSION
Based on the results obtained in this study, the gastroepiploic artery preservation with glycerol as a training model for microvascular anastomosis is a technically very simple procedure and useful for the novice microsurgeon.
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