J Lab Med Qual Assur.
2006 Dec;28(2):239-244.
Comparison of Common Clinical Chemistry Analyte Levels between Greiner Bio-One Vacuette Tube and Glass Tube
- Affiliations
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- 1Department of Laboratory Medicine, College of medicine, The Catholic University of Korea, Seoul, Korea. hkl@catholic.ac.kr
- 2Department of Pathology, College of medicine, The Catholic University of Korea, Seoul, Korea.
- 3Department of Internal Medicine, College of medicine, The Catholic University of Korea, Seoul, Korea.
Abstract
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BACKGROUND: Serum separator tubes were introduced 25 years ago and are widely used in the clinical laboratory for collection of blood. Recently, the plastic serum separator tube has become available for blood collection for lightening and flexibility and suitability for automation. However few studies have been reported on stability of the common analytes in this tube.
METHODS
We evaluated the concentrations of seventeencommonly ordered analytes: aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein, albumin, sodium, chloride, calcium, phosphorus, triglyceride, low density lipoprotein (LDL)-cholesterol, potassium, uric acid, blood urea nitrogen (BUN), total cholesterol, glucose, creatinine in sera separated in plain glass tubes (no gel) and in sera separated in the plastic Greiner Bio-One Vacuette tubes containing serum separator gel (Greiner Bio-One, Kremsm?nster, Austria) by Toshiba 200-FR Neo.
RESULTS
Results were analyzed using two-tailed paired t-tests and Bland-Altman plots. Results from 9 common analytes (glucose, total cholesterol, BUN, potassium, LDL-cholesterol, inorganic phosphorus, calcium, sodium, chloride) were statistically different between glass tube and plastic Greiner Bio-One Vacuette tube, but the differences were not considered to be clinically significant.
CONCLUSIONS
We conclude that the plastic Greiner Bio-One Vacuette tubes are suitable for collection of blood and storage of serum for common analytes.
MeSH Terms
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Alanine Transaminase
Alkaline Phosphatase
Aspartate Aminotransferases
Automation
Blood Urea Nitrogen
Calcium
Calcium Chloride
Chemistry, Clinical*
Cholesterol
Creatinine
Glass*
Glucose
Lipoproteins
Phosphorus
Plastics
Pliability
Potassium
Sodium
Triglycerides
Uric Acid
Alanine Transaminase
Alkaline Phosphatase
Aspartate Aminotransferases
Calcium
Calcium Chloride
Cholesterol
Creatinine
Glucose
Lipoproteins
Phosphorus
Plastics
Potassium
Sodium
Uric Acid
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