J Korean Orthop Res Soc.  1999 Apr;2(1):27-32.

New Chronic Osteomyelitis Model in White Rabbit: A Preliminary Report

• Kang SB
• Lee KB
• Yoon KS
• Lee JH
• Park JS
• Kim DW
• Choi KW
• Kwon IC
• Jeong SY

Abstract

The purpose of this study was to develope a rabbit model for the chronic osteomyelitis, which is reproducible, controllable in quantity of bacteria and suitable for toxicologic research and therapeutic intervention studies. Osteomyelitis was induced in white rabbits by injecting varying numbers of S. aureus(ATCC 19636, 49230) and Alginate-CaCl2 into the proximal metaphysis of tibia. Three rabbits were used in each number of S. aureus respectively. The tibia were harvested at 8 weeks later and evaluation was done by clinical, radiological and histological findings. Clinical sings of infection consisted of soft tissue swelling and limping in rabbits, Radiologic findings were periosteal reaction, osteolysis, new bone formation in proximal tibias. Histology showed chronic active inflammation, debris of alginate, clusters of bacterial and granulation tissue. In ATCC 19636(more than 6X105) inoculated rabbits, osteomyelitis was established consistently in all three rabbits. Using Alginate-CaCl2 and ATCC 19636 Staphylococcus aureus, we made a new chronic osteomyelitis model, reproducible and controllable in quantity of bacteria.

Keyword

Osteomyelitis; Animal model; Rabbit; Alginate

MeSH Terms

Bacteria
Granulation Tissue
Inflammation
Clinical Trial
Models, Animal
Osteogenesis
Osteolysis
Osteomyelitis*
Rabbits
Staphylococcus aureus
Tibia