Influence of synovial Joint Fluid of Rheumatic and Osteoarthritic on Chondrocyte in vitro

Sung, Jin Hyung; Ryu, Jae Duk; Kim, Young Hun; Jung, Kyu Sik; Kim, Weon Yoo; Hwang, Hee Ju; Kim, Jin Young

Abstract

PURPOSE
Earlier work suggested that two cytokines inhibit synthesis of type II collagen and of aggrecans by chondrocytes and they depress chondrocyte proliferation, but there was little report how the chondrocyte is modulated by culture conditions such as the joint fluids of the rheumatoid arthritis and that of the osteoarthritis. The purpose of this investigation was to determine whether RA(rheumatic arthritis) or OA(Osteoarthritis) joint fluid influence proliferation and differentiation in cultured human articular chondrocytes. MATERIALS AND METHODS: Human chondrocytes were cultured in a standard media (DMEM and 10% FBS), RA and OA joint fluid were added to media at the concentration of 20, 40 and 60% respectively for 1, 3 and 6days. 3H-thymidine and 3H-uridine uptake of cultured chondrocytes were measured as indicators of cell proliferation. Synthesis of human collagen type I, II was estimated by the RT-PCR procedures.
RESULTS
3H-thymidine uptake of the chondrocyte cultured in RA SF(synovial fluid) medium at 2 and 4 days; its uptake in the group treated by RA SF 20%, 40%, 60% increased more significantly than that in control group (P<0.05). 3H-thyrnidine uptake of the chondrocyte cultured in OA SF medium at 2 days; its uptake of the group treated in OA SF 60%(P<0.05), but there was no significant difference of its uptake between in the control group & the group treated in OA SF 20%, 40% (P<0.05). 3H-thymidine uptake of the chondrocyte cultured in OA SF medium at 4 days; there was no significant difference of its uptake between control group & OASF treated group(P>0.05). 3H-uridine uptake of the chondrocyte cultured in RA SF medium at 2 and 4 days; its uptake of the group treated by RA SF 20%, 40%, 60% increased more significantly than that of control group (P<0.05). 3H-uridine uptake of the chondrocyte cultured in OA SF medium at 2 days; its uptake of the group treated by OA SF 20%, 40%, 60% increased more significantly than that of control group (P<0.05). 3H-uridine uptake of the chondrocyte cultured in OA SF medium at 4 days; its uptake of the group treated by OA SF 20%, 60% increased more significantly than that of control group (P<0.05), but there was no significant difference of its uptake between control group & OA SF 40% treated group(P>0.05). Human type I collagen mRNA expressions of the chondrocyte markedly increased in RA and OA SF mixed groups. Human type II collagen mRNA expressions of the chondrocyte were reduced in RA and OA SF mixed groups, especially RA SF 60% mixed groups.
CONCLUSION
RA and OA SF increased the proliferation of the articular chondrocyte, but its decreased the differentiation of the chondrocyte. RA and OA SF may change the phenotype of the articular chondrocyte and this phenomenon was more outstanding in RA SF.