J Korean Orthop Res Soc.  2000 Apr;3(1):1-4.

Immunohistochemical Staining for Type II Collagen in Regenerated Cartilage after Microfracture Surgery

• Bae DK
• Chung KW
• Kim JM
• Kwon CH
• Park YK

Abstract

PURPOSE
The purpose of this study is to identify nature of regenerated articular cartilage after microfracture surgery.
MATERIALS AND METHODS
From Oct. 1997 to Nov. 1998, 40 knees were treated for osteoarthritis by arthroscopic microfracture technique. In the 18 knees, during the second arthroscopic procedure, biopsy specimens extending to the subchondral bone were taken and immunohistochemical staining was done to identify type of collagen. One patient was man and 17 patients were women. Average age of the patients were 58 years (range, 40-75 years).
RESULTS
Type II collagen in articular cartilage appeared to be brown color with this staining. Degree of staining were +3 in 4 knees(22%), +2 in 2 knees(11%), +1 in 7 knees(39%) and trace in 5 knees(28%).
CONCLUSION
Microfracture surgery restores the function of the joint by forming predominantly hyaline-like cartilage containing type II collagen. As analyzing amounts of type II collagen with an immunohistochemical staining in regenerated cartilage, we can presume the prognosis of regenerated cartilage tissue after microfracture surgery.

Keyword

Knee; Osteoarthritis; Microfracture; Type II collagen; immunohistochemical; staining

MeSH Terms

Biopsy
Cartilage*
Cartilage, Articular
Collagen
Collagen Type II*
Female
Humans
Joints
Knee
Osteoarthritis
Prognosis
Collagen
Collagen Type II