J Korean Orthop Res Soc.  2005 Apr;8(1):41-49.

Human Muscle Derived Stem Cell Differentiate into Neurons

• Kim YY
• Cho YK
• Kwon SY
• Gi BK
• Song SW
• Kim HJ
• Han CW
• Khang GS

Abstract

PURPOSE
To evaluate the possibility that human muscle derived stem cells (hMDSCs) can be differentiated into neurons in vitro. MATERIAL AND METHODS: Muscle derived stem cells were isolated from the hamstring muscles during the anterior cruciate ligament reconstruction by preplate technique. For the characterization of these cells, desmin staining, CD 34, Sca-1, CD 29 using the Flow cytometry were performed. In the experimental group, neuronal induction media was added to differentiate hMDSCs to neuronal cells. These cells were evaluated by neuronal markers such as neuron-specific enolase (NSE), neurofilament (NF), TrkA using immunocytochemistry. For the control group, no induction media was added. Statstical analyses were performed by use of Kruskal-Wallis H test and Student-Newman-Keuls test (P<0.01). RESULT: Desmin staining was positive in 92.3+/-6%. Flow cytometry was negative for CD 34 and Sca-1. However it was positive for CD 29. (69.4+/-10%). The immunocytochemical result revealed NSE, NF and TrkA positive with 63.2+/-2.3%, 59.2+/-2.5%, 55+/-2.4% respectively. However, these were negative in the control group.
CONCLUSION
Our observations indicate that hMDSCs have the capacity to differentiate into neurons in a specialized culture media.

Keyword

Muscle-derived cell; Neuronal differentiation; NSE; NF; TrkA

MeSH Terms

Anterior Cruciate Ligament Reconstruction
Culture Media
Desmin
Flow Cytometry
Humans*
Immunohistochemistry
Muscles
Neurons*
Phosphopyruvate Hydratase
Stem Cells*
Culture Media
Desmin
Phosphopyruvate Hydratase