J Korean Soc Ther Radiol Oncol.  2001 Sep;19(3):245-251.

Smad6 Gene and Suppression of Radiation-Induced Apoptosis by Genistein in K562 cells

• Jeong SJ
• Jin YH
• Yoo YJ
• Do CH
• Jeong MH
• Huh GY
• Bae HR
• Yang KM
• Moon CW
• Oh SG
• Hur WJ
• Lee HS

Abstract

PURPOSE: The genes involved on the suppression of radiation-induced apoptosis by genistein in K562 leukemia cell line was investigated.
MATERIALS AND METHODS
K562 cells in exponential growth phase were irradiated with a linear accelerator at room temperature. Forx-ray irradiation and drug treatment, cultures were prepared at 2x105 cells/mL. The cells were irradiated with 10 Gy (Clinac 1800C, Varian, USA). Stock solutions of herbimycin A (HMA, Calbiochem, UK) and genistein (Calbiochem, UK) were prepared in dimethylsulfoxide (DMSO, Sigma, UK). After incubation at 37degreesC for 24 h, PCR-select cDNA subtractive hybridization, dot hybridization, DNA sequencing and Northern hybridization were examined.
RESULTS
Smad6 gene was identified from the differentially expressed genes in K562 cells incubated with genistein which had been selected by PCR-select cDNA subtractive hybridization. The mRNA expression of Smad6 in K562 cells incubated with genistein was also higher than control group by Northern hybridization analysis.
CONCLUSION
We have shown that Smad6 involved on the suppression of radiation-induced apoptosis by genistein in K562 leukemia cell line. It is plausible that the relationship between Smad6 and the suppression of radiation-induced apoptosis is essential for treatment development based on molecular targeting designed to modify radiation-induced apoptosis.

Keyword

PTK inhibitors; Oncogene expression; Radiation-induced apoptosis; Smad6

MeSH Terms

Apoptosis*
Cell Line
Dimethyl Sulfoxide
DNA, Complementary
Genistein*
K562 Cells*
Leukemia
Particle Accelerators
RNA, Messenger
Sequence Analysis, DNA
DNA, Complementary
Dimethyl Sulfoxide
Genistein
RNA, Messenger