A study on biocompatability of resin cements for all-ceramic crown

Kim, Kwang Jun; Kim, Sung Hoon; Jin, Tai Ho

Abstract

STATEMENT OF PROBLEM: Resin cements were used widely on all ceramic crowns, but the influence of resin cements on biocells was not understood clearly. PURPOSE: This study was investigated to evaluate the biocompatibility of resin cements for allceramic crowns. MATERIAL AND METHOD: The resin cements used in this study were Panavia F (Kuraray Co., Ltd. Japan), Variolink II (Vivadent Ets., Schann / Liechtenstein), and Bistite II(Bistite dual cure resin cement-clear, Tokuyama Soda Co., Japan). The viability of normal human oral keratocytes, gingival fibroblast, and gingival fibroblast immortalized by Human Papilloma virus 16 was measured in vitro for evaluation of cytotoxicity on resin cements, and the response of pulp tissue was analyzed and evaluated with light microscope after application of cements at cutting edge of incisors. RESULTS: The normal human oral keratocytes was the most sensitive to toxicity of resin cement, and toxicity of cements was higher in Bistite II than in Variolink II. The cell viability of immortalized gingival fibroblast did not affected by type of cement and cultivation period, but there was a tendency that cytotoxicity in Bistite II was higher than in Variolink II. The cell viability of gingival fibroblast was similar to that of immortalized gingival fibroblast regardless of cement type, but Bistite II showed more toxic than others after 5 days cultivation. The responses of pulp tissue according to cement type were similar after 2 days cultivation, but revealed high toxicity in Bistite II after 10 days cultivation. CONCLUSION: Variolink II was more biocompatible than any other resin cements used in this study.