J Korean Soc Emerg Med.  2002 Jun;13(2):141-146.

Effect of Steroids on Interleukin-1beta and Tumor Necrosis Factor-alpha Expression due to High Tidal Volume in the Rat Ventilator-induced Lung Injury Model

• So BH
• Oh DR
• Lee MJ
• Lee WJ
• Kim SK
• Wang YP
• Rha SJ

Abstract

PURPOSE: Although mechanical ventilation is used to improve oxygenation, this strategy can impose injurious mechanical stress on lung tissue. This is recognized as ventilator-induced lung injury (VILI). Recently, several studies have reported that inflammatory cells and mediators play an important role in the progression of VILI. This study was designed to investigate, in a rat model, the expression of interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha, as well as the anti-inflammatory activity of steroids in VILI.
METHODS
Male Sprague-Dawley rats were divided into 3 groups. The control group received low tidal volume (6ml/kg) ventilation. The second group received high tidal volume (25 ml/kg) ventilation and the third group received high tidal volume (25 ml/kg) ventilation with methylprednisolone (30 mg/kg) treatment. After 60 minutes of mechanical ventilation, the animals in each group were sacrificed. Resected lungs were immunostained with antisera for IL-1beta and TNF-alpha. The stained areas were evaluated with an image analyzer.
RESULTS
In the groups that received a high tidal volume, the number of positive pixels in IL-1 beta and TNF-alpha was significantly higher than it was in the low tidal volume group. The high tidal volume group showed greater expression of I L - 1beta and TNF-alpha,but this was significantly decreased by methylprednisolone.
CONCLUSION
Our study suggests that an inflammatory response related with IL-1beta and TNF-alpha is involved in the development of VILI. The expression of IL-1beta and TNF-alpha was lessened by treatment with methylprednisolone, which might have contributed to improving lung dysfunction after mechanical ventilation.

Keyword

Ventilator-induced lung injury; Interleukin-1beta; Tumor necrosis factor-alpha; Methylprednisolone

MeSH Terms

Animals
Humans
Immune Sera
Interleukin-1beta*
Interleukins
Lung
Male
Methylprednisolone
Models, Animal
Oxygen
Rats*
Rats, Sprague-Dawley
Respiration, Artificial
Steroids*
Stress, Mechanical
Tidal Volume*
Tumor Necrosis Factor-alpha*
Ventilation
Ventilator-Induced Lung Injury*
Immune Sera
Interleukin-1beta
Interleukins
Methylprednisolone
Oxygen
Steroids
Tumor Necrosis Factor-alpha