J Korean Assoc Oral Maxillofac Surg.  2006 Feb;32(1):8-18.

Expression of angiogenin, TGF-beta, VEGF, APEX and TNF-alpha in oral squamous cell carcinoma

• Lee HS
• Kim KW
• Kim WJ

Abstract

PURPOSE: The purpose of this study was to verify that the expressions of angiogenin, transforming growth factor-beta(TGF-beta), vascular endothelial growth factor(VEGF), human apurinic/apyrimidinic endonuclease(APEX) and tumor necrosis factoralpha(TNF-alpha) were associated with the tumorigenesis of the oral squamous cell carcinoma(OSCC). MATERIALS AND METHODS: Fifty-one samples of OSCC and fifteen normal oral mucosae were obtained to analyze the expression levels of above five factors. mRNA expressions were quantified by the quantitative competitive PCR(QC-PCR) method. After 2% agarose gel electrophoresis stained with ethidium bromide, the concentration of mRNA was calculated by a digital image analysis system. The expression levels of angiogenin, TGF-beta, VEGF, APEX and TNF-alpha were compared by unpaired Student's ttests between cancer and normal tissues. We analyzed statistically to find the cut-off values that would be useful as diagnostic markers, and the linear regression analysis between every two factors of these five factors by SAS system.
RESULTS
All of these five factors (angiogenin: P<0.0037, TGF-beta: P<0.0001, VEGF: P<0.0102, APEX: P<0.0023, TNF-alpha: P<0.0074) were significantly correlated with OSCC. In the analysis to find the cut-off values for the diagnosis, we could not find any value that had a reasonable sensitivity and specificity. In the linear regression analysis, there were correlations between angiogenin and TNF-alpha, TGF-beta and VEGF, TGF-beta and APEX, TGF-beta and TNF-alpha, VEGF and APEX, VEGF and TNF-alpha, APEX and TNF-alpha.
CONCLUSION
Our results suggest that not only angiogenin, TGF-beta, VEGF, APEX and TNF-alpha are significantly associated with the tumorigenesis, but also the close relationship between these factors might enhance the tumorigenesis of OSCC. We can not find clinical availability for diagnosis.

Keyword

Angiogenin; Transforming growth factor-beta(TGF-beta); Vascular endothelial growth factor(VEGF); Human apurinic/apyrimidinic endonuclease(APEX); Tumor necrosis factor-alpha(TNF-alpha); Oral squamous cell carcinoma(OSCC)

MeSH Terms

Carcinogenesis
Carcinoma, Squamous Cell*
Diagnosis
Electrophoresis, Agar Gel
Ethidium
Humans
Linear Models
Mouth Mucosa
Necrosis
RNA, Messenger
Sensitivity and Specificity
Transforming Growth Factor beta*
Tumor Necrosis Factor-alpha*
Vascular Endothelial Growth Factor A*
Ethidium
RNA, Messenger
Transforming Growth Factor beta
Tumor Necrosis Factor-alpha
Vascular Endothelial Growth Factor A