J Korean Assoc Maxillofac Plast Reconstr Surg.  2009 Jan;31(1):27-34.

Expression of Placenta Growth Factor in the Oral Squamous Cell Carcinoma

• Lee SG
• Kim CH

Abstract

Angiogenesis is essential for solid tumor growth and progression. Among the pro-angiogenetic factors, vascular endothelial growth factor(VEGF), also known as vascular permeability factor, is the most important as a mitogen for vascular endothelium. The VEGF family of molecules currently consists of six growth factors, VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and placenta growth factor(PlGF). Over-expression of PlGF is associated with angiogenesis under pathological conditions such as ischemia, inflammation, and cancer. Hence, the goal of this study is to identify the correlation of clinicopathlogical factors and the up-regulation of PlGF expression in oral squamous cell carcinoma. We studied the immunohistochemical staining of PlGF, PlGF gene expression and a real time quantitative RT-PCR in 20 specimens of 20 patients with oral squamous cell carcinoma. The results were as follows. 1. In the immunohistochemical study of poorly differentiated and invasive oral squamous cell carcinoma, the high level staining of PlGF was observed. And the correlation between immunohistopathological PlGF expression and histological differentiation of specimens was significant (Pearson correlation analysis, significance [r] >0.6, P < .05). 2. In the PlGF gene RT-PCR analysis, PlGF expression was more in tumor tissue than in adjacent normal tissue. Paired-samples analysis determined the difference of PlGF mRNA expression level between the cancer tissue and the normal tissue (Student's t - test, P < .05) These findings suggest that up-regulation of the PlGF gene may play a role in progression and local metastasis in invasive oral squamous cell carcinoma.

Keyword

Placenta growth factor; Squamous cell carcinoma; Vascular endothelial growth factor

MeSH Terms

Carcinoma, Squamous Cell
Endothelium, Vascular
Gene Expression
Humans
Inflammation
Intercellular Signaling Peptides and Proteins
Ischemia
Neoplasm Metastasis
Placenta
Pregnancy Proteins
RNA, Messenger
Up-Regulation
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor B
Vascular Endothelial Growth Factor C
Vascular Endothelial Growth Factor D
Intercellular Signaling Peptides and Proteins
Pregnancy Proteins
RNA, Messenger
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor B
Vascular Endothelial Growth Factor C
Vascular Endothelial Growth Factor D