J Korean Acad Conserv Dent.  2010 Nov;35(6):473-478. 10.5395/JKACD.2010.35.6.473.

Biocompatibility of bioaggregate cement on human pulp and periodontal ligament (PDL) derived cells

• Chung CR
• Kim E
• Shin SJ

• KMID: 2176469
• DOI: http://doi.org/10.5395/JKACD.2010.35.6.473

Abstract

OBJECTIVES
This study was performed to investigate the biocompatibility of newly introduced Bioaggregate on human pulp and PDL cells.
MATERIALS AND METHODS
Cells were collected from human pulp and PDL tissue of extracted premolars. Cell culture plate was coated either with Bioaggregate or white MTA, then the same number of cells were poured to cell culture dishes. Cell attachment and growth was examined under a phase microscope after 1,3 and 7 days of seeding. Cell viability was measured and the data was analyzed using Student t-test and one way ANOVA.
RESULTS
Both types of cells used in this study were well attached and grew healthy on Bioaggregate and MTA coated culture dishes. No cell inhibition zone was observed in Bioaggregate group. There was no statistical difference of viable cells between bioaggreagte and MTA groups.
CONCLUSIONS
Bioaggregate appeared to be biocompatible compared with white MTA on human pulp and PDL cells.

Keyword

Bioaggregate; Biocompatibility; MTA; Periodontal ligament cell; Pulp cell

MeSH Terms

Bicuspid
Calcium Hydroxide
Cell Culture Techniques
Cell Survival
Glutamates
Guanine
Humans
Hydroxyapatites
Periodontal Ligament
Seeds
Silicates
Pemetrexed
Calcium Hydroxide
Glutamates
Guanine
Hydroxyapatites
Silicates

Figure

• 

  Figure 1 Photos taken from an optical microscope (×40 magnification). The same number of cells (a, human pulp cells; b, PDL cells) were seeded to MTA or Bioaggregate coated cell culture dishes, then initial attachment was observed using a phase microscope. PDL, periodontal ligament; MTA, mineral trioxide aggregate.

• 

  Figure 2 Cell viability tests. a, human pulp cells; b, PDL cells. PDL, periodontal ligament; MTA, mineral trioxide aggregate.

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