Blood Res.  2014 Sep;49(3):154-161. 10.5045/br.2014.49.3.154.

Development of NK cell expansion methods using feeder cells from human myelogenous leukemia cell line

Affiliations
  • 1Department of Biology Education, College of Education, Chungbuk National University, Cheongju, Korea. chemokine@cbnu.ac.kr

Abstract

BACKGROUND
Natural killer (NK) cells constantly survey surrounding tissues and remove newly generated cancer cells, independent of cancer antigen recognition. Although there have been a number of attempts to apply NK cells for cancer therapy, clinical application has been somewhat limited because of the difficulty in preparing a sufficient number of NK cells. Therefore, ex vivo NK cell expansion is one of the important steps for developing NK cell therapeutics.
METHODS
CD3+ depleted lymphocytes were cocultured with IL-2 and with feeder cells (peripheral blood mononuclear cells [PBMCs], K562, and Jurkat) for 15 days. Expanded NK cells were tested for cytotoxicity against cancer cell lines.
RESULTS
We compared feeder activities of three different cells-PBMC, K562, and Jurkat. K562 expanded NK cells by almost 20 fold and also showed powerful cytotoxic activity against cancer cells. K562-NK cells remarkably expressed the NK cell activation receptors, NKG2D, and DNAM-1. K562-NK cells exhibited more than two-fold production of cytotoxic granules compared with Jurkat-NK cells, producing more perforin and granzyme B than naive NK cells.
CONCLUSION
Our findings suggest that K562 are more efficient feeder cells than Jurkat or PBMCs. K562 feeder cells expanded NK cells by almost 20 fold and showed powerful cytotoxic activity against cancer cells. We herein propose an intriguing approach for a design of NK cell expansion.

Keyword

Natural killer cells; Feeder cell; K562; Cytotoxic activity; DNAM-1; NKG2D

MeSH Terms

Cell Line*
Feeder Cells*
Granzymes
Humans
Interleukin-2
Killer Cells, Natural*
Leukemia, Myeloid*
Lymphocytes
Perforin
Granzymes
Interleukin-2
Perforin
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