- Engineering Biomaterials for Feeder-Free Maintenance of Human Pluripotent Stem Cells
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Yang K, Lee J, Cho SW
- KMID: 1426150
- Int J Stem Cells.
- 2012 May;5(1):1-5.
Human pluripotent stem cells (hPSCs) are capable of differentiating into any type of somatic cell, a characteristic that imparts significant therapeutic potential. Human embryonic stem cells and induced pluripotent stem... -
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- Growth and Characterization of the Uveal Melanoma in Vitro
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Ko MK, Choe JK, Kim DS
- KMID: 1949761
- J Korean Ophthalmol Soc.
- 1990 Mar;31(3):303-308.
This study was performed to observe the influence of the feeder layer on the culture of the human uveal melanoma cell. Two types of melanoma cells which were obtained from... -
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- Feeder Cells for Culture of Human Embryonic Stem Cells
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Kim YY, Oh SK, Choi YM
- KMID: 2288245
- Korean J Reprod Med.
- 2007 Dec;34(4):219-227.
No abstract available. -
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- Development of NK cell expansion methods using feeder cells from human myelogenous leukemia cell line
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Bae DS, Lee JK
- KMID: 2172786
- Blood Res.
- 2014 Sep;49(3):154-161.
- doi: 10.5045/br.2014.49.3.154
BACKGROUND: Natural killer (NK) cells constantly survey surrounding tissues and remove newly generated cancer cells, independent of cancer antigen recognition. Although there have been a number of attempts to apply... -
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- Maintenance of hPSCs under Xeno-Free and Chemically Defined Culture Conditions
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Lim JJ, Kim HJ, Rhie BH, Lee MR, Choi MJ, Hong SH, Kim KS
- KMID: 2465889
- Int J Stem Cells.
- 2019 Nov;12(3):484-496.
- doi: 10.15283/ijsc19090
Previously, the majority of human embryonic stem cells and human induced pluripotent stem cells have been derived on feeder layers and chemically undefined medium. Those media components related to feeder... -
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- Feeder Cells Free Rabbit Oral Mucosa Epithelial Cell Sheet Engineering
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Oliva J, Ochiai K, Florentino A, Bardag-Gorce F, Wood A, Niihara Y
- KMID: 2411880
- Tissue Eng Regen Med.
- 2018 Jun;15(3):321-332.
- doi: 10.1007/s13770-017-0108-4
The optimal cell culture method of autologous oral mucosal epithelial cell sheet is not well established for a safe transplantation on to the patients' ocular surface. Animal serum and 3T3... -
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- Effect of the Isolation Method of Mouse Inner Cell Mass, Types of Feeder Cells and Treatment Time of Mitomycin C on the Formation Rate of ICM Colony
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Jang HJ, Ko KR, Kim MK, Na YJ, Lee KS
- KMID: 1979176
- Korean J Fertil Steril.
- 2006 Dec;33(4):265-272.
OBJECTIVE: This study was carried out to evaluate the effect of the isolation methods of inner cell mass from mouse blastocyst, types of feeder cells and treatment time of mitomycin... -
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- The Use of Canine Bone-Marrow Stromal Cell Line, DO64, as Feeder Cells for The Efficient Cloning of Human B Lymphoblastoid Cell Lines ( B-LCLs )
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Kim TG, Han H, Kim YS, Cho HI
- KMID: 1550780
- Korean J Immunol.
- 1999 Jun;21(2):109-113.
Epstein-Barr virus (EBV) is a potent inducer of polyclonal B lymphocyte proliferation and a tool for the establishment of human B lymphoblastoid cell lines (B-LCLs), which have proven useful for... -
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- Developmental Competence of Buffalo (Bubalus bubalis) Pluripotent Embryonic Stem Cells Over Different Homologous Feeder Layers and the Comparative Evaluation with Various Extracellular Matrices
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Sharma M, Dubey PK, Kumar R, Nath A, Kumar GS, Sharma GT
- KMID: 1426173
- Int J Stem Cells.
- 2013 May;6(1):26-36.
BACKGROUND AND OBJECTIVES: Use of somatic cells as a feeder layer to maintain the embryonic stem cells (ESCs) in undifferentiated state limits the stem cell research design, since experimental data... -
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- Human Amniotic Fluid Cells Support Expansion Culture of Human Embryonic Stem Cells
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Kim HS, Seol HW, Ahn HJ, Oh SK, Ku SY, Kim SH, Choi YM, Kim JG, Moon SY
- KMID: 2235683
- Korean J Fertil Steril.
- 2004 Dec;31(4):261-272.
OBJECTIVE: This study was performed to evaluate the possibility of prolonged culture of human embryonic stem cells (hESC; SNUhES2) on human amniotic fluid cells (hAFC), which had been storaged after... -
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- Establishment of Human Embryonic Stem Cells using Mouse Embryonic Fibroblasts and Human Fetal Fibroblasts as Feeder Cells
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Cho HW, Ko KR, Kim MK, Lee JI, Sin SI, Lee DH, Kim KH, Lee KS
- KMID: 2085171
- Korean J Fertil Steril.
- 2005 Jun;32(2):133-147.
OBJECTIVES: This study was carried out to establish human embryonic stem cells derived from frozen-thawed embryos using mouse embryonic fibroblasts (mEFs), human fetal skin and muscle fibroblasts as feeder cells,... -
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- Overexpression of bone morphogenetic protein 4 in STO fibroblast feeder cells represses the proliferation of mouse embryonic stem cells in vitro
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Kim GH, Lee GR, Choi HI, Park NH, Chung HT, Han IS
- KMID: 1397450
- Exp Mol Med.
- 2012 Jul;44(7):457-463.
Embryonic stem cells (ESCs) can be propagated in vitro on feeder layers of mouse STO fibroblast cells. The STO cells secrete several cytokines that are essential for ESCs to maintain... -
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- Establishment and Characterization of an In Vitro Model for Cholesteatoma
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Raynov AM, Choung YH, Park HY, Choi SJ, Park K
- KMID: 1486059
- Clin Exp Otorhinolaryngol.
- 2008 Jun;1(2):86-91.
- doi: 10.3342/ceo.2008.1.2.86
OBJECTIVES: Experimental models are of importance to study the pathogenesis of middle ear cholesteatoma, however, they were not established until now. We aimed to develop in vitro model of middle... -
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- Telomerase: Key to Mortal or Immortal Road
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Yang EY, Sung YH, Lee HW
- KMID: 1974515
- Immune Netw.
- 2002 Dec;2(4):183-188.
- doi: 10.4110/in.2002.2.4.183
Gradual attrition of telomere to a critical short length elicits successive cellular response of cellular senescence and crisis. Cancer cells evade this process by maintaining functional telomeres via one of... -
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- Induced Differentiation of Embryonic Stem Cells to Insulin Secreting Cells
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Sung JH, Lim CK, Choi HW, Lee HS, Shin H, Jun JH, Yoon HS, Koong MK
- KMID: 2235677
- Korean J Fertil Steril.
- 2004 Dec;31(4):209-216.
OBJECTIVE: Embryonic stem (ES) cells could be differentiated into the specific cell types by alternation of culture condition and modification of gene expression. This study was performed to evaluate the... -
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- Establishment and Characterization of Multipotent Germ Line Stem Cells (MGSCs) from Neonatal Mouse Testis
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Han SC, Song H, Jun JH
- KMID: 1483347
- Korean J Reprod Med.
- 2008 Mar;35(1):39-48.
OBJECTIVE: The aim of this study was to investigate whether multipotent germline stem cells (MGSCs) can be established from neonatal mouse testis. METHODS: Various cells containing MGSCs were collected from neonatal... -
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- A Study on Recovery from Potentially Lethal Damage induced by gamma-Irradiation in Plateau-phase Vero Cells in vitro
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Kim IH, Choi EK, Ha SW, Park CI, Cha CY
- KMID: 1657605
- J Korean Soc Ther Radiol.
- 1988 Jun;6(1):1-12.
Recovery from potentially lethal damage (PLDR) after irradiation was studied in plateau-phase culture of Vero cells in vitro. Unfed plateau-phase cells were irradiated with dose of 1 to 9 gy... -
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- Establishment of Mouse Embryonic Stem Cell-like Cells from In Vitro Fertilized Embryos
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Moon SY, Park YB, Kim HS, Oh SK, Chun DW, Suh CS, Choi YM, Kim JG, Lee JY, Kim SH
- KMID: 1979162
- Korean J Fertil Steril.
- 2002 Mar;29(1):1-12.
OBJECTIVE: In order to acquire the technique for the establishment of human embryonic stem cells (ESC) derived from the human frozen-thawed embryos produced in IVF-ET program, this study was performed... -
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- Establishment of Embryonic Stem Cell Line from Isolated Blastomeres from Mouse Preimplantation Embryos
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Lim CK, Sung JH, Choi HW, Cho JW, Shin MR, Jun JH
- KMID: 1511387
- Korean J Fertil Steril.
- 2006 Mar;33(1):25-34.
OBJECTIVE: The aim of this study was to investigate whether embryonic stem (ES) cells can be established from isolated blastomeres of mouse embryos. METHODS: Blastomeres were separated from mouse (C57Bl/6J) 2-... -
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- Effects of Keratinocyte Growth Factor (KGF), Epidermal Growth Factor (EGF), and Extracellular Calcium on the Growth of Cultured Psoriatic Keratinocytes
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Kim KH, Kim JY, Yoon TJ, Kim HS, Lee CW, Eim JJ
- KMID: 2302607
- Korean J Dermatol.
- 2005 Jun;43(6):732-742.
We investigated the effects of KGF and EGF, together with extracellular calcium, on the growth of cultured psoriatic keratinocytes, in order to establish a new chemically defined medium for culturing... -
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