Chonnam Med J.  2001 Dec;37(4):377-382.

Interrelationship between Oxidative Neuronal Injury Induced by Iron or Buthionine Sulfoximine and NF-kappaB Activity in Mixed Cortical Cultures

• Lee KS
• Bae CS
• Kim JK

Abstract

In order to delineate the interrelationship between oxidative neuronal injury and changes in NF-kappaB activity, the effects of trolox (a vitamin E analog antioxidant), cycloheximde (CHX, a protein synthesis inhibitor) or pyrrolidine dithiocarbamate (PDTC, a inhibitor of NF-kappaB activation) on the neuronal death and the changes in NF-kappaB binding activity induced by FeCl2 or buthionine sulfoximine (BSO) were examined in mouse mixed cortical cultures. The neuronal death induced by 20 micrometer FeCl2 was almost abolished by co-treatment with trolox (100 micrometer), but markedly potentiated by co-treatment with CHX (1 microgram/ml) or PDTC (50 micrometer). The neuronal death induced by 1 mM BSO was almost abolished by co-treatment with trolox or CHX, but not affected by co-treatment with PDTC (50 micrometer). NF-kappaB binding activity was induced in nuclear extract of cells exposed for 2 hr to 20 micrometer FeCl2 or 1 mM BSO. Co-treatment with CHX potentiated the induction of NF-kappaB binding activity by the exposure to 20 micrometer FeCl2 for 2 hr, but prevented the induction of NF-kappaB binding activity by 1 mM BSO. Trolox or PDTC prevented the induction of NF-kappaB binding activity by FeCl2 or BSO. These results demonstrated that the changes of NF-kappaB binding activity took part in the effects of CHX or trolox on the oxidative neuronal death as well as the oxidative neuronal death induced by FeCl2 or BSO.

Keyword

Oxidative neuronal death; NF-kappaB binding activity; Cortical culture; Cycloheximide

MeSH Terms

Animals
Buthionine Sulfoximine*
Cycloheximide
Iron*
Mice
Neurons*
NF-kappa B*
Vitamin E
Vitamins
Buthionine Sulfoximine
Cycloheximide
Iron
NF-kappa B
Vitamin E
Vitamins