Ann Dermatol.  2016 Apr;28(2):186-191. 10.5021/ad.2016.28.2.186.

Efficacy of Red or Infrared Light-Emitting Diodes in a Mouse Model of Propionibacterium acnes-Induced Inflammation

• Lee WJ
• Lee KC
• Kim MJ
• Jang YH
• Lee SJ
• Kim DW

• KMID: 2171375
• DOI: http://doi.org/10.5021/ad.2016.28.2.186

Abstract

BACKGROUND
Laser/light-based devices may provide an alternative to conventional acne therapeutics in some patients with nonresponsive acne.
OBJECTIVE
We investigated the efficacy of red or infrared light-emitting diode (LED) devices in a mouse model of Propionibacterium acnes-induced inflammation through clinical examination and histopathological and immunohistochemical studies.
METHODS
A human-derived Propionibacterium acnes suspension (10(9) colony-forming units /µl) was injected into the back of an HR-1 mouse. Then, a 28.9 J/cm2 650-nm red LED or 9.3 J/cm2 830-nm infrared LED was applied to the mouse with P. acnes-induced inflammation once daily for 2 weeks. Two weeks after treatment, histological findings with hematoxylin and eosin staining and expression levels of inflammatory biomarkers (integrin α6, neutrophils, interleukin [IL]-1β, and matrix metalloproteinase [MMP]-2/9) were evaluated in tissue specimens using immunohistochemical staining.
RESULTS
Mice treated with red and infrared LED showed clinical improvement in inflammatory nodules compared to mice in the control group. Red LED was much more effective than infrared LED. Epidermal hyperplasia, comedone-like cysts, and integrin α6 expression improved to a similar extent in the red and infrared LED treatment groups and control group. Neutrophil, IL-1β, MMP-2, and MMP-9 expression after treatment with red and infrared LED decreased considerably compared to expression in the control group.
CONCLUSION
In a mouse model of P. acnes-induced inflammatory nodules, red and infrared LED devices may be an alternative to conventional acne therapies. In addition, a mouse model of P. acnes-induced inflammatory nodules is helpful for laboratory research of acne.

Keyword

Acne; Light; Mice; Propionibacterium acnes

MeSH Terms

Acne Vulgaris
Animals
Biomarkers
Eosine Yellowish-(YS)
Hematoxylin
Humans
Hyperplasia
Inflammation*
Interleukins
Mice*
Neutrophils
Propionibacterium acnes
Propionibacterium*
Stem Cells
Eosine Yellowish-(YS)
Hematoxylin
Interleukins

Figure

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  Fig. 1 Clinical inflammatory lesions in group B and group C improved greatly compared to those in group A.

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  Fig. 2 Improvement in epidermal hyperplasia and microcomedone-like cysts in group B and group C was similar to that in group A.

• 

  Fig. 3 Integrin α6 expression in group B and group C was similar to that in group A.

• 

  Fig. 4 Neutrophil infiltration decreased in group B and group C compared to that in group A.

• 

  Fig. 5 Interleukin-1β expression decreased in group B and group C compared to that in group A.

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  Fig. 6 Matrix metalloproteinase (MMP)-2 and MMP-9 expression decreased greatly in group B and group C compared to that in group A.

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