Int J Oral Biol.  2011 Jun;36(2):103-108.

Characteristics of New Estrogen Biosensor Employing Taste Principles

• Kwon SB
• Lee CH
• Kim KN

Abstract

Measurement of estrogen concentration in bio-samples are very important for differential diagnosis of various disease or evaluation of health status. However, it is difficult to collect immediate data of estrogen concentration because they are measured by radioimmunoassay or chromatography which need time- and cost-consuming sample pre-treatment. This study was performed for development of new estrogen biosensor employing taste principles, and for evaluation of cross reactivity between various steroid hormones. Gene sequence of ligand binding domain of alpha-human estrogen receptor (amino acid 302-553; hER-LBD) was cloned from human breast cancer cell line. The proteins of hER-LBD were produced by T7-E.coli expression system, and isolated by chromatography. hER-LBD were coated on the gold plated quartz crystal (AT-cut 9MHz), and resonance frequencies were measured by universal frequency counter. Estradiol, progesterone, testosterone, and aldosterone were used for cross reactivity of the hER-LBD. We also monitored influences of pH change in resonance frequency. The resonance frequencies of hER-LBD coated quartz crystal were decreased during increase of estrogen concentration from 15 microg/mL to 50 microg/mL. However, similar steroid hormones, progesterone and aldosterone, did not elicit the change in resonance frequency. Testosterone evoke weak change in resonance frequency. The new estrogen biosensor was more sensitive in pH 7.2 than in pH 7.6. These results suggest that hER-LBD coated quartz crystal biosensor is a probable estrogen biosensor.

Keyword

taste; biosensor; estrogen; receptor; endocrine disrupting chemicals

MeSH Terms

Aldosterone
Biosensing Techniques
Breast Neoplasms
Cell Line
Chromatography
Clone Cells
Collodion
Diagnosis, Differential
Endocrine Disruptors
Estradiol
Estrogens
Humans
Hydrogen-Ion Concentration
Organothiophosphorus Compounds
Progesterone
Proteins
Quartz
Radioimmunoassay
Testosterone
Aldosterone
Collodion
Endocrine Disruptors
Estradiol
Estrogens
Organothiophosphorus Compounds
Progesterone
Proteins
Quartz
Testosterone