Biomol Ther.  2014 Jul;22(4):334-340.

Anti-Inflammatory and PPAR Transactivational Effects of Oleanane-Type Triterpenoid Saponins from the Roots of Pulsatilla koreana

• Li W
• Yan XT
• Sun YN
• Ngan TT
• Shim SH
• Kim YH

Abstract

In this study, 23 oleanane-type triterpenoid saponins were isolated from a methanol extract of the roots of Pulsatilla koreana. The NF-kappaB inhibitory activity of the isolated compounds was measured in TNFalpha-treated HepG2 cells using a luciferase reporter system. Compounds 19-23 inhibited TNFalpha-stimulated NF-kappaB activation in a dose-dependent manner, with IC50 values ranging from 0.75-8.30 microM. Compounds 19 and 20 also inhibited the TNFalpha-induced expression of iNOS and ICAM-1 mRNA. Moreover, effect of the isolated compounds on PPARs transcriptional activity was assessed. Compounds 7-11 and 19-23 activated PPARs the transcriptional activity significantly in a dose-dependent manner, with EC50 values ranging from 0.9-10.8 microM. These results suggest the presence of potent anti-inflammatory components in P. koreana, and will facilitate the development of novel anti-inflammatory agents.

Keyword

Pulsatilla koreana; NF-kappaB inhibitory activity; Tumor necrosis factor-alpha; PPAR

MeSH Terms

Anti-Inflammatory Agents
Hep G2 Cells
Inhibitory Concentration 50
Intercellular Adhesion Molecule-1
Luciferases
Methanol
NF-kappa B
Peroxisome Proliferator-Activated Receptors*
Pulsatilla*
RNA, Messenger
Saponins*
Tumor Necrosis Factor-alpha
Anti-Inflammatory Agents
Intercellular Adhesion Molecule-1
Luciferases
Methanol
NF-kappa B
Peroxisome Proliferator-Activated Receptors
RNA, Messenger
Saponins
Tumor Necrosis Factor-alpha