Infect Chemother.  2006 Jun;38(3):146-153.

Evaluation of a New Diagnostic Test for Invasive Fungal Infections Using Prophenoloxydase System to Measure (1->3)-beta-D-glucan Concentration

Affiliations
  • 1Department of Internal Medicine, Eulji University School of Medicine, Daejeon, Korea.
  • 2Samyang Genex Food and Bio Research Center, Daejeon, Korea.
  • 3Regional Innovation Center, Seowon University, Cheongju, Korea.
  • 4College of Pharmacy, Pusan National University, Busan, Korea.
  • 5Department of Internal Medicine, Dongguk University College of Medicine, Gyeongju, Korea.
  • 6Clinical Research Institute, Seoul National University Hospitial, Seoul, Korea.
  • 7Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea. mdohmd@snu.ac.kr

Abstract

BACKGROUND: Prognosis of invasive fungal infection is supposed to be better when diagnosed earlier. (1->3)-beta-D-glucan (BDG) test is considered useful in early diagnosis of invasive fungal infections among high-risk patients. A new diagnostic test using prophenoloxydase system to measure BDG level is developed. A study was performed to evaluate clinical usefulness of this new diagnostic test.
MATERIALS AND METHODS
15 patients of confirmed invasive fungal infections and 38 healthy normal volunteers were enrolled. Plasma or serum BDG concentrations were measured using prophenoloxydase system. Assays for intra-run variability and inter-run variability were performed. A cut-off value was determined and sensitivity and specificity of the test were evaluated.
RESULTS
A cut-off value of 94.90 pg/mL was determined. Sensitivity and specificity of the test were 86.7% and 52.6%, respectively. Statistical analyses of inter-run variability and intra-run variability revealed the test is reliable (P< or =0.001).
CONCLUSION
BDG test using prophenoloxydase system is a sensitive and reliable test in non-invasive detection of invasive fungal infection.

Keyword

Invasive fungal infection; Non-invasive diagnosis; (1-->3)-beta-D-glucan; Prophenoloxydase system

MeSH Terms

Diagnostic Tests, Routine*
Early Diagnosis
Healthy Volunteers
Humans
Plasma
Prognosis
Sensitivity and Specificity
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