J Biomed Res.  2014 Mar;15(1):1-6.

Therapeutic effects of combination of Galla rhois extract and Sodium chlorate on Mice infected with Brucella abortus

• Cha CN
• Hong IH
• Yu EA
• Park EK
• Yoo CY
• Kim S
• Lee HJ

Abstract

This study investigated the therapeutic effects of Galla rhois (GR) ethanol extract (GRE), sodium chlorate (SC), and a combination of GRE and SC on mice infected with Brucella abortus (B. abortus). Mice were infected intraperitoneally with B. abortus and then treated with GRE, SC, and a combination GRE and SC in drinking water for 14 days. Then, serum antibodies were used in a tube agglutination test (TAT), after which the weight and CFUs from each spleen were measured. In addition, histopathological changes in each liver were examined at 14 days post-infection. At 14 days post-infection, negative reactions of serum antibodies in PC (positive control), SCT (SC 1.6 g/L drinking water), GRT (GRE 200 mg/L drinking water), and GST (GRE 200 mg + SC 1.6 g/L drinking water) were 0, 40, 60, and 80%, respectively. The average spleen weight was not significantly different between the groups. At 14 days post-infection, bacterial numbers in all treated groups were significantly lower compared to to that of the PC (GRT and SCT, P<0.05; GST, P<0.001). In terms of histopathological changes in the livers, there were numerous multifocal microgranulomas in the PC, whereas this number successively decreased in the SCT, GRT, and GST groups. Conclusively, a combination of GRE and SC exhibits therapeutic effects on mice infected with B. abortus. These results suggest the potential efficacy of a mixture of GRE and SC in the treatment of brucellosis.

Keyword

Brucella abortus; Galla rhois; sodium chlorate; combination; mice

MeSH Terms

Agglutination Tests
Animals
Antibodies
Brucella abortus*
Brucellosis
Drinking
Drinking Water
Ethanol
Liver
Mice*
Sodium*
Spleen
Antibodies
Drinking Water
Ethanol
Sodium