Clin Exp Otorhinolaryngol.  2016 Mar;9(1):44-50. 10.21053/ceo.2016.9.1.44.

The Healing Effects of Autologous Mucosal Grafts in Experimentally Injured Rabbit Maxillary Sinuses

• Topdag M
• Kara A
• Konuk E
• Demir N
• Ozturk M
• Calıskan
• Topdag DO
• Ulubil A
• Keskin IG
• Iseri M

• KMID: 2166286
• DOI: http://doi.org/10.21053/ceo.2016.9.1.44

Abstract

OBJECTIVES
Healing processes of the nose and paranasal sinuses are quite complex, and poorly understood. In this study, we aimed to compare the effect of mucosal autologous grafts on the degenerated rabbit maxillary sinus mucosa with spontaneous wound healing. It is hypothesized that mucosal grafts will enhance ciliogenesis and improve the morphology of regenerated cilia.
METHODS
Ten female New Zealand rabbits were included in the study. They underwent external maxillary sinus surgery through a transcutaneous approach. A total of 20 maxillary sinuses were randomly divided into 2 groups: 'spontaneous healing group' and 'autologous graft group.' The animals were sacrificed at the 14th day after the surgery. Scanning electron microscope (SEM), and light microscope were used for the evaluation.
RESULTS
Cellular composition of the graft group is better than the spontaneous healing group. The graft group had larger areas covered with ciliary epithelium than the spontaneous healing group, and the mean length of the cilias were also longer. Additionally, there were wider cilia with abnormal morphology areas in the spontaneous healing group.
CONCLUSION
In our opinion, covering of the denuded areas with a graft improves re-epithelization, and may prevent the early complications after sinus surgeries.

Keyword

Nasal Mucosa; Maxillary Sinus; Rabbits

MeSH Terms

Animals
Cilia
Epithelium
Female
Humans
Maxillary Sinus*
Mucous Membrane
Nasal Mucosa
Nose
Paranasal Sinuses
Rabbits
Transplants*
Wound Healing

Figure

• 

  Fig. 1. Anterior walls of the maxillary sinuses (A), bilaterally bone flaps (B), elevation of an approximately 1-cm2 mucosal area on the medial maxillary wall (C), healed animal (D), healed anterior wall of the sinus (E), inflamed and hypertrophic mucosa with neo osteogenesis (F).

• 

  Fig. 2. Light microscopic examinations (toluidin blue) showed graft group (A) contained more and higher epithelial cells than spontaneous healing group (B).

• 

  Fig. 3. Scanning electron microscope images of epithelial ciliary organizations. Graft (A) and spontaneous healing group (B). Apical surface buds are seen early of the epitelial ciliary development both of the graft and spontaneous healing groups. They are showed with ring in the A and B panels.

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  Fig. 4. Cell cordons in the graft group were spreaded over the surface to form a superficial cover; graft (A, B), epithelial dispersion was in different areas and unable to create a cover; spontaneous healing group (C, D).

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  Fig. 5. Abnormal ciliary formations in the scanning electron microscope images. Graft (A) and spontaneous healing group (B).

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