Cancer Res Treat.  2006 Feb;38(1):1-6.

Tissue Array Methods for High-throughput Clinicopathologic Research

• Lee HS
• Kim WH

Abstract

Recent research in molecular biology has identified a significant number of novel markers, which may have diagnostic, prognostic and therapeutic significance. High-throughput tissue array method facilitates the validation of novel markers by enabling the simultaneous analysis of hundreds or thousands of tissue specimens. Tissue array slides can be analyzed using techniques such as immunohistochemistry and in situ hybridization. In this review, we give a brief overview of tissue array method and its application to high throughput clinicopathologic research.

Keyword

Tissue array; Translational research; Immunohistochemistry; In situ hybridization

MeSH Terms

Immunohistochemistry
In Situ Hybridization
Molecular Biology
Translational Medical Research

Figure

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  Fig. 1 Construction of tissue array blocks. (A) The preparatory work before construction, (B) Donor block, recipient block, and construction of tissue array block.

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  Fig. 2 Tissue array slides with diverse core size.

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  Fig. 3 Profile of DNA-PKcs expression status in various malignant neoplasms using multi-tumor arrays.

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