Korean J Blood Transfus.  2016 Apr;27(1):31-37. 10.17945/kjbt.2016.27.1.31.

Usefulness of Additional LISS/Coombs Card Test with Enzyme-Treated Red Cells in Detecting Anti-Kidd Antibodies Not Detectable by NaCl/Enzyme Card Test Alone

• Chu D
• Park SJ
• Seo SW
• Yang HJ
• Chung Y
• Kwon SW

• KMID: 2163118
• DOI: http://doi.org/10.17945/kjbt.2016.27.1.31

Abstract

BACKGROUND
Detection of anti-Kidd antibody is important because of its clinical significance. If detection is difficult due to weak serological reactivity or dosage effect, use of an enzyme method could be helpful. However, despite use of an enzyme method, we still observed weak reactivity of anti-Kidd antibody.
METHODS
All identified anti-Kidd antibody cases from Jan 2012 to Aug 2015 in Asan Medical Center were reviewed. Antibody identification test was performed using the column agglutination technique using Bio-Rad ID-DiaPanel with LISS/Coombs card, Bio-Rad ID-DiaPanel-P with NaCl/Enzyme card, and ID-DiaPanel-P with LISS/Coombs card. The test results were compared.
RESULTS
Sixty cases of anti-JK(a) or anti-Jk(b) were detected and tested by enzyme method. Among them, 34 (56.6%) cases showed strengthened reactivity using the ID-DiaPanel-P with NaCl/Enzyme card method. However, 26 (43.4%) cases showed weakened reactivity. Of these, 13 cases that could be tested by an additional method using ID-DiaPanel-P with LISS/Coombs card containing anti-IgG and anti-C3d showed successfully strengthened reactivity.
CONCLUSION
The reactivity of anti-Kidd antibodies that was not strengthened using ID-DiaPanel-P with NaCl/Enzyme card method could be successfully strengthened by use of the ID-DiaPanel-P with LISS/Coombs card.

Keyword

Enzyme-treated red cell; Unexpected antibody; Anti-Kidd antibody; Anti-C3d antibody; LISS/Coombs card

MeSH Terms

Agglutination
Antibodies*
Chungcheongnam-do
Antibodies