Ann Surg Treat Res.  2016 Apr;90(4):213-217. 10.4174/astr.2016.90.4.213.

Effect of ankaferd blood stopper in experimental peritoneal adhesion model

• Karaca G
• Aydin O
• Pehlivanli F
• Kocael A
• Pekcici R
• Duymus E
• Akgedik S
• Guler O

• KMID: 2160951
• DOI: http://doi.org/10.4174/astr.2016.90.4.213

Abstract

PURPOSE
Ankaferd Blood Stopper (ABS) is an herbal extract attained from 5 different plants. It has the therapeutic potential to be used for the management of external hemorrhage and controlling gastrointestinal bleedings. To date, the safety of ABS for intraperitoneal usage is not clear. In this study, we investigated the effectiveness and safety of using intraperitoneal ABS in an experimental peritoneal adhesion model.
METHODS
Twenty-four male Wistar Albino rats were used in the study. The rats were randomly divided into 3 groups: saline, ABS, and control. On the 10th day, all rats were euthanized. The adhesions were evaluated by Nair's macroscopic adhesion classification, and pathologically evaluated with Zühlke's microscopic adhesion classification.
RESULTS
macroscopic and microscopic comparison between the ABS and saline groups did not show any differences but both the ABS and saline groups were superior when compared to the control group.
CONCLUSION
ABS was found equally effective with saline on the abdominal adhesions and to no effect on postoperative adhesion formation.

Keyword

Ankaferd blood stopper; Rats; Intra-abdominal adhesion; Peritoneum

MeSH Terms

Animals
Classification
Hemorrhage
Humans
Male
Peritoneum
Rats

Reference

1. Davey AK, Maher PJ. Surgical adhesions: a timely update, a great challenge for the future. J Minim Invasive Gynecol. 2007; 14:15–22.

2. Aysan E, Basak E, Kinaci F, Yanar H, Coskun H. Experimental adhesion model: effect of viscosities of fluids put in the peritoneal cavity on preventing peritoneal adhesions. Exp Anim. 2007; 56:349–354.

3. Tarhan OR, Barut I, Sutcu R, Akdeniz Y, Akturk O. Pentoxifylline, a methyl xanthine derivative, reduces peritoneal adhesions and increases peritoneal fibrinolysis in rats. Tohoku J Exp Med. 2006; 209:249–255.

4. Muller SA, Treutner KH, Haase G, Kinzel S, Tietze L, Schumpelick V. Effect of intra-peritoneal antiadhesive fluids in a rat peritonitis model. Arch Surg. 2003; 138:286–290.

5. Gungor G, Goktepe MH, Biyik M, Polat I, Tuna T, Ataseven H, et al. Efficacy of ankaferd blood stopper application on non-variceal upper gastrointestinal bleeding. World J Gastrointest Endosc. 2012; 4:556–560.

6. Kurt M, Disibeyaz S, Akdogan M, Sasmaz N, Aksu S, Haznedaroglu IC. Endoscopic application of ankaferd blood stopper as a novel experimental treatment modality for upper gastrointestinal bleeding: a case report. Am J Gastroenterol. 2008; 103:2156–2158.

7. Shorbagi A, Sivri B. Successful management of a difficult case of radiation proctopathy with Ankaferd BloodStopper: a novel indication (with video). Gastrointest Endosc. 2010; 72:666–667.

8. Turhan N, Kurt M, Shorbagi A, Akdogan M, Haznedaroglu IC. Topical Ankaferd Blood Stopper administration to bleeding gastrointestinal carcinomas decreases tumor vascularization. Am J Gastroenterol. 2009; 104:2874–2877.

9. Tasdelen Fisgin N, Tanriverdi Cayci Y, Coban AY, Ozatli D, Tanyel E, Durupinar B, et al. Antimicrobial activity of plant extract Ankaferd Blood Stopper. Fitoterapia. 2009; 80:48–50.

10. Kilic O, Gonen M, Acar K, Yurdakul T, Avunduk MC, Esen HH, et al. Haemostatic role and histopathological effects of a new haemostatic agent in a rat bladder haemorrhage model: an experimental trial. BJU Int. 2010; 105:1722–1725.

11. Kalayci MU, Soylu A, Eroglu HE, Kubilay D, Sancak B, Ugurluoglu C, et al. Effect of ankaferd blood stopper on hemostas is and histopathological score in experimental liver injury. Bratisl Lek Listy. 2010; 111:183–188.

12. Huri E, Haznedaroglu IC, Akgul T, Astarci M, Ustun H, Germiyanoulu C. Biphasic effects of ankaferd blood stopper on renal tubular apoptosis in the rat partial nephrectomy model representing distinct levels of hemorrhage. Saudi Med J. 2010; 31:864–868.

13. Elkins TE, Bury RJ, Ritter JL, Ling FW, Ahokas RA, Homsey CA, et al. Adhesion prevention by solutions of sodium carboxymethylcellulose in the rat. I. Fertil Steril. 1984; 41:926–928.

14. Nair SK, Bhat IK, Aurora AL. Role of proteolytic enzyme in the prevention of postoperative intraperitoneal adhesions. Arch Surg. 1974; 108:849–853.

15. Zuhlke HV, Lorenz EM, Straub EM, Savvas V. Pathophysiology and classification of adhesions. Langenbecks Arch Chir Suppl II Verh Dtsch Ges Chir. 1990; 1009–1016.

16. Holmdahl L, Risberg B, Beck DE, Burns JW, Chegini N, diZerega GS, et al. Adhesions: pathogenesis and prevention-panel discussion and summary. Eur J Surg Suppl. 1997; (577):56–62.

17. Holmdahl L, al-Jabreen M, Risberg B. Experimental models for quantitative studies on adhesion formation in rats and rabbits. Eur Surg Res. 1994; 26:248–256.

18. Rasa K, Erverdi N, Karabulut Z, Renda N, Korkmaz A. The effect of methylene blue on peritoneal adhesion formation. Turk J Gastroenterol. 2002; 13:108–111.

19. Kluger Y, Weinbroum A, Ben-Avraham R, Galili Y, Klausner J, Rabau M. Reduction in formation of peritoneal adhesions by methylene blue in rats: a dose response study. Eur J Surg. 2000; 166:568–571.

20. Comert M, Karakaya K, Barut F, Karadeniz Cakmak G, Ucan HB, Gultekin FA, et al. Does intraabdominal use of Ankaferd Blood Stopper cause increased intraperitoneal adhesions? Ulus Travma Acil Cerrahi Derg. 2010; 16:383–389.

21. Kandemir O, Buyukates M, Kandemir NO, Aktunc E, Gul AE, Gul S, et al. Demonstration of the histopathological and immunohistochemical effects of a novel hemostatic agent, Ankaferd Blood Stopper, on vascular tissue in a rat aortic bleeding model. J Cardiothorac Surg. 2010; 5:110.

22. Elkelani OA, Molinas CR, Mynbaev O, Koninckx PR. Prevention of adhesions with crystalloids during laparoscopic surgery in mice. J Am Assoc Gynecol Laparosc. 2009; 9:447–452.

23. Larsson B, Lalos O, Marsk L, Tronstad SE, Bygdeman M, Pehrson S, et al. Effect of intraperitoneal instillation of 32% dextran 70 on postoperative adhesion formation after tubal surgery. Acta Obstet Gynecol Scand. 1985; 64:437–441.

24. Isler SC, Demircan S, Cakarer S, Cebi Z, Keskin C, Soluk M, et al. Effects of folk medicinal plant extract Ankaferd Blood Stopper on early bone healing. J Appl Oral Sci. 2010; 18:409–414.