Gut Liver.  2015 Jul;9(4):486-493. 10.5009/gnl14040.

The Effects of Broccoli Sprout Extract Containing Sulforaphane on Lipid Peroxidation and Helicobacter pylori Infection in the Gastric Mucosa

• Chang YW
• Jang JY
• Kim YH
• Kim JW
• Shim JJ

• KMID: 2160632
• DOI: http://doi.org/10.5009/gnl14040

Abstract

BACKGROUND/AIMS
The aims of this study were to investigate whether a broccoli sprout extract containing sulforaphane (BSES) inhibited the Helicobacter pylori infection density and exerted an antioxidative effect on gastric mucosal damage.
METHODS
The enrolled subjects were randomized in a double-blinded manner into three groups. Finally, 33 H. pylori (+) BSES treatment subjects (group A), 28 H. pylori (+) placebo subjects (group B), and 28 H. pylori (-) BSES treatment subjects (group C) were studied. H. pylori infection density was indirectly quantified by a 13C-urea breath test (UBT), and the ammonia concentration in gastric juice aspirates was measured through gastroscopic examination. Malondialdehyde (MDA), an oxidative damage biomarker, and reduced glutathione (GSH), an antioxidant biomarker, were measured in the gastric mucosa by an enzyme-linked immunosorbent assay.
RESULTS
BSES treatment did not significantly affect the UBT values or ammonia concentration in group A (p=0.634 and p=0.505, respectively). BSES treatment did significantly reduce mucosal MDA concentrations in group A (p<0.05) and group C (p<0.001), whereas the gastric mucosal GSH concentrations did not differ before and after treatment in any of the groups.
CONCLUSIONS
BSES did not inhibit the H. pylori infection density. However, BSES prevented lipid peroxidation in the gastric mucosa and may play a cytoprotective role in H. pylori-induced gastritis.

Keyword

Helicobacter pylori; Sulforaphane; Malondialde-hyde; Glutathione

MeSH Terms

Adult
Ammonia/metabolism
Antioxidants/*pharmacology
Biomarkers/analysis
Brassica/*chemistry
Breath Tests
Double-Blind Method
Enzyme-Linked Immunosorbent Assay
Female
Gastric Juice/enzymology
Gastric Mucosa/*drug effects/metabolism
Glutathione/analysis
Helicobacter Infections/*drug therapy
*Helicobacter pylori
Humans
Isothiocyanates/*pharmacology
Lipid Peroxidation/*drug effects
Male
Malondialdehyde/analysis
Middle Aged
Plant Extracts/chemistry/*pharmacology
Urea
Ammonia
Antioxidants
Biomarkers
Glutathione
Isothiocyanates
Malondialdehyde
Plant Extracts
Urea