J Korean Med Sci.  2015 Jun;30(6):705-709. 10.3346/jkms.2015.30.6.705.

Serum Specific IgE to Thyroid Peroxidase Activates Basophils in Aspirin Intolerant Urticaria

• Shin YS
• Suh DH
• Yang EM
• Ye YM
• Park HS

• KMID: 2160597
• DOI: http://doi.org/10.3346/jkms.2015.30.6.705

Abstract

Thyroid antibodies are frequently observed in urticaria patients, but their roles in urticaria are not clearly elucidated. We investigated the role of serum specific IgE to thyroid peroxidase (TPO) in patients with aspirin intolerant acute urticaria (AIAU) and aspirin intolerant chronic urticaria (AICU). We recruited 59 AIAU and 96 AICU patients with 69 normal controls (NC). Serum specific IgE to TPO was measured by manual direct ELISA, and CD203c expressions on basophil with additions of TPO were measured to prove a direct role of TPO in effector cells. The prevalences of serum specific IgE to TPO were significantly higher in AIAU (15.2%) and AICU groups (7.5%) compared to NC (0%, P=0.018: P=0.013, respectively). Flow cytometry showed CD203c induction in a dose dependent manner with serial additions of TPO in some AIAU and AICU patients having high specific IgE to TPO. Our findings show that the prevalence of serum specific IgE to TPO was significantly higher in both AIAU and AICU patients than in NC. It is suggested that specific IgE to TPO play a pathogenic role in AIAU and AICU.

Keyword

Urticaria; Autoimmunity; Thyroid Peroxidase Antibody; Aspirin Intolerance; Basophil Activation Test

MeSH Terms

Adult
Anti-Inflammatory Agents, Non-Steroidal/adverse effects
Aspirin/*adverse effects
Autoantibodies/immunology
Basophils/drug effects/*immunology
Humans
Immunoglobulin E/blood/*immunology
Iodide Peroxidase/blood/*immunology
Urticaria/*chemically induced/*immunology/pathology
Anti-Inflammatory Agents, Non-Steroidal
Aspirin
Autoantibodies
Immunoglobulin E
Iodide Peroxidase

Figure

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  Fig. 1 The prevalence of serum specific IgE to TPO. Serum specific IgE to TPO was measured by manual direct ELISA system from AIAU (n = 59), AICU (n = 96), and NC (n = 69) groups. The cut-off value for serum specific IgE to TPO level was defined as mean and three standard deviation of healthy controls. AIAU, aspirin intolerant acute urticaria; AICU, aspirin intolerant chronic urticaria; NC, normal control; TPO, thyroid peroxidase.

• 

  Fig. 2 Expression level of CD203c on basophils. Peripheral blood basophils were obtained from two subjects with high serum specific IgE to TPO and healthy control. The expression of CD203c on basophil were measured with the additions of TPO to prove a direct role of specific IgE to TPO on effector cells of urticaria. AIAU, aspirin intolerant acute urticaria; AICU, aspirin intolerant chronic urticaria; NC, normal control; TPO, thyroid peroxidase.

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