Lab Med Online.  2016 Apr;6(2):111-115. 10.3343/lmo.2016.6.2.111.

Bacteroides nordii and Bacteroides salyersiae Isolated from Post-operative Peritonitis Patients

• Yoon JG
• Kim H
• Lee Y
• Kim M
• Yong D
• Jeong SH
• Lee K

• KMID: 2160094
• DOI: http://doi.org/10.3343/lmo.2016.6.2.111

Abstract

In this study, we report three cases in which two species of the Bacteroides fragilis group, 'Bacteroides nordii' and 'Bacteroides salyersiae', were isolated from peritoneal fluid cultures from post-operative peritonitis patients. The two species of the B. fragilis group were initially misidentified as B. fragilis/Bacteroides stercoris and Bacteroides ovatus by Rapid ID 32A (bioMérieux, France), and finally confirmed as B. nordii and B. salyersiae using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16s rRNA sequencing. For the identification of anaerobes, particularly B. fragilis group organisms, MALDI-TOF MS is a useful method not only because of its concordance with 16S rRNA sequencing results, but also because of its rapidity and simple procedure.

Keyword

Bacteroides fragilis group; Bacteroides nordii; Bacteroides salyersiae; Spectrometry; Mass; Matrix-assisted laser desorption-ionization

MeSH Terms

Ascitic Fluid
Bacteroides fragilis
Bacteroides*
Humans
Mass Spectrometry
Peritonitis*
Spectrum Analysis

Reference

1. Eija K, William GW, Diane MC. Bacteroides, Porphyromonas, Prevotella, Fusobacterium, and other anaerobic gram-negative rods. In : Versalovic J, editor. Manual of clinical microbiology. 10th ed. Washington, D.C.: ASM Press;2011.

2. Sears CL. A dynamic partnership: celebrating our gut flora. Anaerobe. 2005; 11:247–251.

3. Wexler HM. Bacteroides: the good, the bad, and the nitty-gritty. Clin Microbiol Rev. 2007; 20:593–621.

4. Jousimies-Somer HR. Update on the taxonomy and the clinical and laboratory characteristics of pigmented anaerobic gram-negative rods. Clin Infect Dis. 1995; 20:Suppl 2. S187–S191.

5. Sakamoto M, Benno Y. Reclassification of Bacteroides distasonis, Bacteroides goldsteinii and Bacteroides merdae as Parabacteroides distasonis gen. nov., comb. nov., Parabacteroides goldsteinii comb. nov. and Parabacteroides merdae comb. nov. Int J Syst Evol Microbiol. 2006; 56:1599–1605.

6. Hardham JM, King KW, Dreier K, Wong J, Strietzel C, Eversole RR, et al. Transfer of Bacteroides splanchnicus to Odoribacter gen nov as Odoribacter splanchnicus comb. nov., and description of Odoribacter denticanis sp. nov., isolated from the crevicular spaces of canine periodontitis patients. Int J Syst Evol Microbiol. 2008; 58:103–109.

7. Goldstein EJ, Citron DM, Goldman PJ, Goldman RJ. National hospital survey of anaerobic culture and susceptibility methods: III. Anaerobe. 2008; 14:68–72.

8. Bakir MA, Kitahara M, Sakamoto M, Matsumoto M, Benno Y. Bacteroides finegoldii sp. nov., isolated from human faeces. Int J Syst Evol Microbiol. 2006; 56:931–935.

9. Bakir MA, Kitahara M, Sakamoto M, Matsumoto M, Benno Y. Bacteroides intestinalis sp. nov., isolated from human faeces. Int J Syst Evol Microbiol. 2006; 56:151–154.

10. Bakir MA, Sakamoto M, Kitahara M, Matsumoto M, Benno Y. Bacteroides dorei sp. nov., isolated from human faeces. Int J Syst Evol Microbiol. 2006; 56:1639–1643.

11. Kim MS, Roh SW, Bae JW. Bacteroides faecis sp. nov., isolated from human faeces. Int J Syst Evol Microbiol. 2010; 60:2572–2576.

12. Lee Y, Kim HS, Yong D, Jeong SH, Lee K, Chong Y. Bacteroides faecis and Bacteroides intestinalis recovered from clinical specimens of human intestinal origin. Yonsei Med J. 2015; 56:292–294.

13. Song YL, Liu CX, McTeague M, Finegold SM. "Bacteroides nordii" sp. nov. and "Bacteroides salyersae" sp. nov. isolated from clinical specimens of human intestinal origin. J Clin Microbiol. 2004; 42:5565–5570.

14. Clinical and. CLSI document M11-A8. Pennsylvania. 2012; CLSI.

15. Nagy E, Maier T, Urban E, Terhes G, Kostrzewa M. Species identification of clinical isolates of Bacteroides by matrix-assisted laser-desorption/ ionization time-of-flight mass spectrometry. Clin Microbiol Infect. 2009; 15:796–802.

16. Simmon KE, Mirrett S, Reller LB, Petti CA. Genotypic diversity of anaerobic isolates from bloodstream infections. J Clin Microbiol. 2008; 46:1596–1601.

17. Coltella L, Mancinelli L, Onori M, Lucignano B, Menichella D, Sorge R, et al. Advancement in the routine identification of anaerobic bacteria by MALDI-TOF mass spectrometry. Eur J Clin Microbiol Infect Dis. 2013; 32:1183–1192.

18. Culebras E, Rodríguez-Avial I, Betriu C, Gómez M, Picazo JJ. Rapid identification of clinical isolates of Bacteroides species by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry. Anaerobe. 2012; 18:163–165.

19. Gardiner BJ, Tai AY, Kotsanas D, Francis MJ, Roberts SA, Ballard SA, et al. Clinical and microbiological characteristics of Eggerthella lenta bacteremia. J Clin Microbiol. 2015; 53:626–635.

20. Venugopal AA, Szpunar S, Johnson LB. Risk and prognostic factors among patients with bacteremia due to Eggerthella lenta. Anaerobe. 2012; 18:475–478.

21. Ko SH, Chiang KL, Kao HW, Chen LC, Ng CJ, Chen PY, et al. Crystallization in transfer set before continuous ambulatory peritoneal dialysis initiation-three case reports. Perit Dial Int. 2014; 34:244–245.

22. Lee Y, Park Y, Kim MS, Yong D, Jeong SH, Lee K, et al. Antimicrobial susceptibility patterns for recent clinical isolates of anaerobic bacteria in South Korea. Antimicrob Agents Chemother. 2010; 54:3993–3997.