J Korean Med Sci.  2003 Jun;18(3):392-396. 10.3346/jkms.2003.18.3.392.

The Pattern of Differentially Expressed Genes in Biliary Atresia

• Choe BH
• Kim KM
• Kwon S
• Lee KS
• Koo JH
• Lee HM
• Kim MK
• Kim JC

• KMID: 2157656
• DOI: http://doi.org/10.3346/jkms.2003.18.3.392

Abstract

Biliary atresia is a progressive obliterative cholangiopathy, but the etiology of this disorder remains uncertain. Identifying genes specifically expressed in biliary atresia and analyzing the pattern of expression may lead to a better understanding of the pathogenesis. Liver tissues were taken from a recipient with biliary atresia and a normal donor during liver transplantation. Total RNA was extracted from each sample and reversely transcribed to cDNA. Then radiolabeled cDNA probe pools were made by random primed DNA labeling method and used for screening of differentially expressed genes by hybridizing with expressed sequence tags (EST) dot blot panel. Northern blot hybridization was done to confirm that these genes are also differentially expressed in other liver tissues. Among 1,730 EST clones, 26 cDNA clones were significantly overexpressed in biliary cirrhosis, while 2 clones were significantly decreased in biliary atresia. By Northern blot hybridization, the results of tissue inhibitor of metalloproteinase (TIMP)-1 and IGFBP-2 were well correlated with differential EST screening (DES). This study identified the pattern of differentially expressed genes in the biliary cirrhosis due to biliary atresia using DES technique.

Keyword

Genes; Biliary Atresia; Liver Cirrhosis; Expressed Sequence Tags

MeSH Terms

Biliary Atresia/*genetics
Blotting, Northern
Gene Expression Profiling/*methods
Gene Library
Human
Insulin-Like Growth Factor-Binding Protein 2/genetics
Tissue-Inhibitor of Metalloproteinase-1/genetics