J Korean Med Sci.  2001 Oct;16(5):623-629. 10.3346/jkms.2001.16.5.623.

Detection of Telomerase Activity in Psoriasis Lesional Skin and Correlaton with Ki-67 Expression and Suppression by Retinoic Acid

• Jang HS
• Oh CK
• Jo JH
• Kim YS
• Kwon KS

• KMID: 2157573
• DOI: http://doi.org/10.3346/jkms.2001.16.5.623

Abstract

Telomerase activity is usually detected in most tumor tissues but not in normal tissues. Recently, there is increasing evidence that telomerase activity is associated with cell proliferation without malignancy, whereas there is little information about telomerase activity and its relationship with cell proliferation in chronic hyperproliferative skin diseases. Thus, we studied telomerase activity in skins from 10 patients with psoriasis and compared telomerase activity with the expression of Ki-67, a proliferation marker, using immunohistochemical staining. The effect of retinoic acid on the telomerase activity in HaCaT cells was also evaluated. Telomerase activity was detected in 7 (70%) of 10 lesional skins of psoriasis and none of the nonlesional skin. Telomerase activity in lesional skin was significantly associated with Ki-67 labelling index. Retinoic acid treatment on HaCaT cells inhibited telomerase activity, which correlated with inhibition of cell proliferation by the agent. The results of our study represent another example that shows telomerase activity correlates with cellular proliferation. Further studies on the regulation of the telomerase are needed to understand the cellular factors involved in controlling telomerase activity.

Keyword

Cell proliferation; HeCaT Cells; Ki-67 Antige; Psoriasis; Tretinoin; Telomerase

MeSH Terms

Cell Division/drug effects
Cell Line
Enzyme Inhibitors/*pharmacology
Human
Ki-67 Antigen/*analysis
Psoriasis/*enzymology
Skin/*enzymology
Telomerase/antagonists & inhibitors/*metabolism
Tretinoin/*pharmacology