Immune Netw.  2011 Dec;11(6):364-367. 10.4110/in.2011.11.6.364.

Production of Prostaglandin E2 and I2 Is Coupled with Cyclooxygenase-2 in Human Follicular Dendritic Cells

• Cho W
• Kim J
• Cho KB
• Choe J

• KMID: 2150721
• DOI: http://doi.org/10.4110/in.2011.11.6.364

Abstract

BACKGROUND
Prostaglandins (PGs) play pathogenic and protective roles in inflammatory diseases. The novel concept of PGs as immune modulators is being documented by several investigators. By establishing an in vitro experimental model containing human follicular dendritic cell-like cells, HK cells, we reported that HK cells produce prostaglandin E2 (PGE2) and prostaglandin I2 (PGI2) and that these PGs regulate biological functions of T and B cells.
METHODS
To investigate the respective contribution of cyclooxygenase-1 (COX-1) and COX-2 to PGE2 and PGI2 production in HK cells, we performed siRNA technology to knock down COX enzymes and examined the effect on PG production.
RESULTS
Both PGE2 and PGI2 productions were almost completely inhibited by the depletion of COX-2. In contrast, COX-1 knockdown did not significantly affect PG production induced by lipopolysaccharide (LPS).
CONCLUSION
The current results suggest that mPGES-1 and PGIS are coupled with COX-2 but not with COX-1 in human follicular dendritic cell (FDC) and may help understand the potential effects of selective COX inhibitors on the humoral immunity.

Keyword

Human; Stromal cells; Lipid mediator

MeSH Terms

Cyclooxygenase 1
Cyclooxygenase 2
Dendritic Cells, Follicular
Dinoprostone
Epoprostenol
Humans
Immunity, Humoral
Intramolecular Oxidoreductases
Models, Theoretical
Prostaglandins
Research Personnel
RNA, Small Interfering
Stromal Cells
Cyclooxygenase 1
Cyclooxygenase 2
Dinoprostone
Epoprostenol
Intramolecular Oxidoreductases
Prostaglandins
RNA, Small Interfering

Figure

• 

  Figure 1 COX-1 and COX-2 proteins were markedly depleted in HK cells by siRNA technology. Control, COX-1, or COX-2 siRNA-transfected HK cells (1×105 cells) were cultured for 48 h, followed by further incubation in the presence or absence of LPS (1µg/ml) for 8 h. The depletion degree of target proteins was evaluated by immunoblotting. β-actin was used to demonstrate equal loading of lysates. The results were reproducible in three independent experiments.

• 

  Figure 2 Depletion of COX-2 but not COX-1 prevents LPS-induced production of PGE2 and PGI2. Control, COX-1, or COX-2 siRNA-transfected HK cells (1×105 cells) were cultured for 48 h, followed by further incubation in the presence or absence of LPS (1µg/ml) for another 48 h. The amounts of PGE2 (A) and 6-keto-PGF1α (B) in the culture supernatants were measured by EIA and normalized by protein concentration in each well. Data are presented as means±standard error of the mean (SEM) of duplicates. Representative results of three reproducible experiments. Asterisks indicate significant differences (*p <0.05, **p<0.01). NS, non-significant.

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