Korean J Vet Res.  2015 Dec;55(4):241-246. 10.14405/kjvr.2015.55.4.241.

Establishment of a live vaccine strain against fowl typhoid and paratyphoid

• Cho SH
• Ahn YJ
• Kim TE
• Kim SJ
• Huh W
• Moon YS
• Lee BH
• Kim JH
• Kwon HJ

• KMID: 2148067
• DOI: http://doi.org/10.14405/kjvr.2015.55.4.241

Abstract

To develop a live vaccine strain against fowl typhoid and paratyphoid caused by Salmonella serovar Gallinarum biovar Gallinarum (Salmonella Gallinarum) and Salmonella serovar Enteritidis (Salmonella Enteritidis), respectively, several nalidixic acid resistant mutants were selected from lipopolysaccharide (LPS) rough strains of Salmonella Gallinarum that escaped from fatal infection of a LPS-binding lytic bacteriophage. A non-virulent and immunogenic vaccine strain of Salmonella Gallinarum, SR2-N6, was established through in vivo pathogenicity and protection efficacy tests. SR2-N6 was highly protective against Salmonella Gallinarum and Salmonella Enteritidis and safer than Salmonella Gallinarum vaccine strain SG 9R in the condition of protein-energy malnutrition. Thus, SR2-N6 may be a safe and efficacious vaccine strain to prevent both fowl typhoid and paratyphoid.

Keyword

attenuation; fowl typhoid; live vaccine; paratyphoid; rough strain

MeSH Terms

Bacteriophages
Nalidixic Acid
Protein-Energy Malnutrition
Salmonella
Salmonella enteritidis
Typhoid Fever*
United Nations
Virulence
Nalidixic Acid