J Korean Soc Microbiol.
1997 Feb;32(1):1-14.
Molecular Epidemiologic Analysis of Nosocomial Escherichia coli Isolates
Abstract
- One hundred and eighteen strains of Escherichia coli isolated from clinical specimens were epidemiologically analyzed for antimicrobial resistance, EcoRI restriction endonuclease analysis, southern hybridization with TEM and SHV probe of conjugative R plasmids. 1. Sixty-two to 73% of E. coli isolates were resistant to ampicillin, carbenicillin, sulfisomidine, and tetracycline, and 20-27% to kanamycin, gentamicin, tobramycin, and nalidixic acid. However more than 93% were susceptible to cephalosporins and all strains were highly susceptible to cefotetan, imipenem, aztreonam, and amikacin. 2. Twelve strains were susceptible to all drugs tested and the multiple resistant strains showed 65 resistance pattern types. 3. Thirty-six resistant strains(34%) transferred R plasmids to E. coli RG488 or RG176 by mixed culture. Fifty-six plasmids with 31 different resistant phenotype were obtained from them. 4. Some of 15 plasmids derived from 10 strains showed identical or similar EcoRI restriction endonuclease digestion patterns, hybridized fragment patterns with TEM probe by southern hybridization, and resistance levels of j3-lactams and aminoglycosides. These results indicate that the epidemic strains or plasmids were present in this hospital and molecular genetic analysis of R plasmids can be used to discriminate clinical isolates of multi- resistant E. coli.
MeSH Terms
-
Amikacin
Aminoglycosides
Ampicillin
Aztreonam
Carbenicillin
Cefotetan
Cephalosporins
Digestion
DNA Restriction Enzymes
Escherichia coli*
Escherichia*
Gentamicins
Imipenem
Kanamycin
Molecular Biology
Nalidixic Acid
Phenotype
Plasmids
R Factors
Sulfisomidine
Tetracycline
Tobramycin
Amikacin
Aminoglycosides
Ampicillin
Aztreonam
Carbenicillin
Cefotetan
Cephalosporins
DNA Restriction Enzymes
Gentamicins
Imipenem
Kanamycin
Nalidixic Acid
Sulfisomidine
Tetracycline
Tobramycin
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