Korean J Dermatol.  1987 Jun;25(3):361-365.

Immunohistologic Differentiation Between Bullous Pemphigoid and Epidermolysis Bullosa Acquisita

• Lee CW

Abstract

Due to the limited availability of immunoelectron microscopy, an alternative method for the differentiation between anti-lamina lucida and anti-sublamina densa antibodies was introduced; indirect immunofluorescence using NaCl-treated human skin as the substrate. In this study author examined sera and lesional skin of 4 cases of bullous pemphigoid (BP), and 2 cases of epidermolysis bulloaa acquisita(EBA) with the above mentioned indirect imrnunofluorescence and modified direct immunofluorescence to evaluate the specificity of the tests. The results showed that in BP the fluorescence patterns were epidermal in 3 patients with 1 combined by indirect immunofluorescence, and epidermal in all 4 patients by modified direct immunofluorescence. In ERA the fluorescence were dermal patterns in both 2 patients by indirect and modified direct immunofluorescence. These data are further confirming the syecificity and the reproducibility of the NaCl extraction technique for the irnmunofluorescence to differentiate the localization of the autoantibodies in the above two bullous dermatoses.

Keyword

Anti - BMZ antibodies; NaCl - separated skin; Immunofluorescence

MeSH Terms

Antibodies
Autoantibodies
Epidermolysis Bullosa Acquisita*
Epidermolysis Bullosa*
Fluorescence
Fluorescent Antibody Technique
Fluorescent Antibody Technique, Direct
Fluorescent Antibody Technique, Indirect
Humans
Microscopy, Immunoelectron
Pemphigoid, Bullous*
Sensitivity and Specificity
Skin
Skin Diseases, Vesiculobullous
Antibodies
Autoantibodies