Korean J Otolaryngol-Head Neck Surg.  2002 Sep;45(9):884-888.

Anticancer Effect and Apoptosis Mechanism of Photochemotherapy Using Photogem and 632 nm Diode Laser on Human Squamous Cell Carcinoma Cell Line

• Chung PS
• Kim HG
• Lee JG
• Lee CH
• Oh CH
• Ahn JC

Abstract

BACKGROUND AND OBJECTIVES: This study was conducted to investigate the anticancer effect of photochemotherapy in vitro using a photosensitizing agent (Photogem) and a laser therapy (632 nm diode laser). MATERIALS AND METHOD: Human squamous cell carcinoma cell (SNU-1041) was treated to laser therapy for various irradiation times, laser powers, and interval times. The treated cell was analyzed by MTT assay, DAPI staining to see apoptosis.
RESULTS
The viability of cells was decreased with the increasing of the laser irradiation time and the laser power. No significant difference in cell viability was noted by various interval time. Increasing apoptosis was observed by increasing concentration of Photogem and increasing lasering time by DAPI staining.
CONCLUSION
This study demonstrated anticancer effect of photochemotherapy using Photogem and 632 nm diode laser. Apoptosis was observed in the process of cancer cell death.

Keyword

Photosensitizing agent; Laser therapy; Photochemotherapy; Apoptosis

MeSH Terms

Apoptosis*
Carcinoma, Squamous Cell*
Cell Death
Cell Line*
Cell Survival
Humans*
Laser Therapy
Lasers, Semiconductor*
Photochemotherapy*