Tuberc Respir Dis.
2004 Aug;57(2):125-131. 10.4046/trd.2004.57.2.125.
Construction of Recombinant BCGs Overexpressing Antigen 85 Complex and Their Protective Efficacy against Mycobacterium tuberculosis Infection in a Mouse Model
- Affiliations
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- 1Department of Molecular Biology, Korean Institute of Tuberculosis, Seoul, Korea. gbai@hotmail.com
- 2Department of Microbiology, Yonsei University College of Medicine, Seoul, Korea.
- 3Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Korea.
- 4Department of Biochemistry and Molecular Biology, College of Science Technology, Hanyang University, Ansan, Korea.
- KMID: 2132493
- DOI: http://doi.org/10.4046/trd.2004.57.2.125
Abstract
- Tuberculosis (TB) remains an enormous global health problem, and a new vaccine against TB more potent than the current inadequate BCG vaccine is urgently needed. We constructed three recombinant Mycobacterium bovis BCG (rBCG) strains over-expressing antigen (Ag) 85A, Ag85B, or both of M. tuberculosis using their own promoter and secretory sequence, or hsp60 promoter. SDS-PAGE analysis of rBCG proteins showed over-expression of Ag85A and Ag85B proteins in higher level than of those in their parental strain of BCG. In addition, rBCG(rBCG/B.FA) over-expressing Ag85A and Ag85B induced strong IFN-gamma production in splenocytes. However, there was no significant difference in protective efficacy between rBCG and their parental BCG strain. In this study, therefore, rBCG over-expressing Ag85A, Ag85B, or both failed to show enhanced protection against M. tuberculosis infection in a mouse model.
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