Tuberc Respir Dis.  2005 Jan;58(1):54-58. 10.4046/trd.2005.58.1.54.

Expression of Estrogen and Progesterone Receptors in Non- small-cell Lung Cancer Tissue Using Tissue Microarray Method

Affiliations
  • 1Department of Pathology, College of Medicine, Konkuk University, Seoul, Korea.
  • 2Department of Internal Medicine, College of Medicine, Inha University, Incheon, Korea. jsryu@inha.ac.kr
  • 3Department of Chest Surgery, College of Medicine, Inha University, Incheon, Korea.

Abstract

BACKGROUND: To evaluate the role of estrogen and progesterone in the carcinogenesis of NSCLC, IHC studies for the expression of the receptors of estrogen and pro?gesterone have been performed with inconsistent results. Recently the TMA method has been developed and has become recognized as a useful and rapid method for extensively analysing molecular markers at the gene and protein level. We have investigated their expressions in the tissue from NSCLC using the microarray method.
METHODS
The TMA construction was made with 70 formalin- fixed, paraffin-embedded tissues of NSCLC. After heat-induced epitope retrieval, IHC staining on pri?mary tissues of NSCLC was performed with the monoclonal antibodies, ER1D5 and PR1A6.
RESULTS
Our sample of 70 consisted of 74% men and 26% women. Of the patients, 49% were current smokers, 27% were non-smokers and 24% were former smokers. By histologic classification, 34 patients had squamous cell carcinoma, 24 had adenocarcinoma, 9 had adenosquamous cell carcinoma, and 3 had other carcinomas. No cancer cells were immunostained with these monoclonal antibodies in any primary tissues of NSCLC.
CONCLUSIONS
No expression of neither of the two receptors was found in any of the lung cancer tissues. This su?ggests that adequate genetic variants for IHC staining need to be developed for NSCLC.

Keyword

Lung Cancer; Receptor; Estrogen; Progesterone

MeSH Terms

Adenocarcinoma
Antibodies, Monoclonal
Carcinogenesis
Carcinoma, Squamous Cell
Classification
Estrogens*
Female
Humans
Lung Neoplasms*
Lung*
Male
Progesterone*
Receptors, Progesterone*
Antibodies, Monoclonal
Estrogens
Progesterone
Receptors, Progesterone
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