Korean J Obstet Gynecol.  1998 Oct;41(10):2571-2575.

Determination of Interleukin-1beta and Transforming Growth Factor-beta1 Levels in Early Human Embryo Coculture-Conditioned Media

• Ha JW
• Lee S
• Hur EJ
• Park JW
• Lee WK

Abstract

This experiment was carried out to determine if 4~8 cell staged human embryo can produce IL-1beta and TGF-beta1, and to evaluate the probable benefit of co-culture system in the setting of IVF-ET by measuring the concentrations of these cytokines in the media of coculture system. The subjects were 10 pregnant and 20 non-pregnant women undergoing IVF-ET with GnRH long protocol, and 167 normally fertilized eggs were cocultured. The concentrations of IL-1beta and TGF-beta1 are measured by ELISA method in the following three kinds of media; TCM media containing 15% fetal cord serum (control media), TCM media culturing cumulus cells for 32~34 hours and the coculture media of fertilized eggs and cumulus cells for 32~34 hours. The mean concentrations of IL-1beta and TGF-beta1 in each media were as followings: 65.5+/-17.0 ng/ml and 17.6+/-1.0 ng/ml in control media, 74.0+/-21.3 ng/ml and 18.2+/-1.2 ng/ml in cumulus containing media, and 73.7+/-21.1 ng/ml and 17.9+/-1.3 ng/ml in study media, respectively. These data show that 4~8 cell stage human embryo does not significantly produce IL-1beta and TGF-beta1 and there was no difference in pregnant and nonpregnant group. Also, These data show that there may be no evidence for positive effect of coculture system such as production of embryotrophic factors.

Keyword

Interleukin-1beta; Transforming growth factor-beta1; Embryo; Coculture

MeSH Terms

Coculture Techniques
Cumulus Cells
Cytokines
Embryonic Structures*
Enzyme-Linked Immunosorbent Assay
Female
Gonadotropin-Releasing Hormone
Humans*
Interleukin-1beta*
Transforming Growth Factor beta1
Zygote
Cytokines
Gonadotropin-Releasing Hormone
Interleukin-1beta
Transforming Growth Factor beta1