J Korean Ophthalmol Soc.  2000 Oct;41(10):2085-2089.

Endotoxin of Gram Positive Bacteria Induced Acute Uveitis in the Rat

• Kim JS
• Shin BM
• Joo MJ

Abstract

The purpose of this study is to examine the capacity of lipoteichoic acid (LTA) to induce intraocular inflammation in the rat. LTAs, obtained from Staphylococcus aureus, Streptococcus pyogenes, and Streptococcus faecalis, were suspended in saline solution in 15 mg/kg concentration and injected into both footpads of female Lewis rats. Lipopolysaccharide (LPS) was injected in the same way. It has been known that LPS induced acute uveitis experimentally in the rat. The uveitis changes were assessed by protein concentration in the aqueous humor and pupil size after injection. Twenty four hours after administration, the protein concentration of LPS injected rats was 667.00+/-45.13 mg/dl, and those of LPA injected rats Staphylococcus aureus, Streptococcus pyogenes, and Streptococcus faecalis were 167.67+/-17.75 mg/dl, 63.00+/-8.85 mg/dl, and 53.93+/- 13.76 mg/dl, respectively. Anterior uveitis induced by LTAs was weaker than that induced by LPS. This model may be useful for investigation of Gram-positive bacterial infection and uveitis.

Keyword

Anterior uveitis; Lipopolysaccharide; Lipoteichic acid

MeSH Terms

Animals
Aqueous Humor
Enterococcus faecalis
Female
Gram-Positive Bacteria*
Gram-Positive Bacterial Infections
Humans
Inflammation
Pupil
Rats*
Sodium Chloride
Staphylococcus aureus
Streptococcus pyogenes
Uveitis*
Uveitis, Anterior
Sodium Chloride