J Korean Ophthalmol Soc.  1997 Jun;38(6):969-975.

Endotoxin Induced Uveitis Model

• Kim MH
• Kim HS
• Joo CK

Abstract

Endotoxin-induced uveitis model was produced in Lewis rats by footpad injection of Salmonella endotoxin(lipopolysaccharide, LPS). The clinical course and histological examination were observed at intervals of two hours. Immunohistochemical staining of intercellular adhesion molecule-1(ICAM-1) and lymphoyte function associated antigen-1(LFA-1) were also peformed. Initial intraocular inflammatory signs were observed at 8-10 hours after injection. Clinical and histological abnormalities peaked at 24-48 hours and were resolving after 72 hours. Histological changes were limited to anterior uvea. ICAM-1 expression was first noted on cells of iris and ciliary body and LFA-1 was expressed on infiltring inflammatory cells 8 hours after the injection and increased by 24 hours and disappeared after 72 hours. LPS induced uveitis in the rat provides a simple, reproducible model for anterior uveitis. ICAM-1 and LFA-1 expression in uveal tract are increased during early phase of uveitis and may enhance the adherence of inflammatory cells with the subsequent initiation of inflammation.

Keyword

Endotoxin; ICAM-1; lipopolysacharide; LFA-1; Uveitis

MeSH Terms

Animals
Ciliary Body
Inflammation
Intercellular Adhesion Molecule-1
Iris
Lymphocyte Function-Associated Antigen-1
Rats
Salmonella
Uvea
Uveitis*
Uveitis, Anterior
Intercellular Adhesion Molecule-1
Lymphocyte Function-Associated Antigen-1