J Korean Ophthalmol Soc.  2001 Feb;42(2):378-385.

Stromelysin Gene Expression in Rat Eye Mediated by the Adenovirus Vector

• Kang S
• Hur WH
• Kee C

Abstract

The purpose of this study is to evaluate the eligibility of gene therapy in treating glaucoma by constructing a replication deficient recombinant adenovirus carrying cDNA of stromelysin and by observing its expression in the rat eye. Stromelysin cDNA was obtained by RT-PCR using total RNA extracted from the cultured human trabecular cells. The cDNA was cloned into TA vector and subsequently into p deltaA. CMV. pA. The adenovirus vector containing stromelysin cDNA was constructed by co-transfection of pJM17 and p delta A. CMV-str. pA into the 293 cells. The expression of stromelysin in the trabecular meshwork and uveoscleral outflow channel of the rat eyes was detected by in situ hybridization and immunohistochemistry. In conclusion, this study showed the possibility of gene therapy in glaucoma by showing the expression of stromelysin with recombinant adenovirus vector containing stromelysin cDNA in the rat eye.

Keyword

Adenovirus vector; Gene therapy; Glaucoma; In vivo study; Stromelysin

MeSH Terms

Adenoviridae*
Animals
Clone Cells
DNA, Complementary
Gene Expression*
Genetic Therapy
Glaucoma
Humans
Immunohistochemistry
In Situ Hybridization
Matrix Metalloproteinase 3*
Rats*
RNA
Trabecular Meshwork
DNA, Complementary
Matrix Metalloproteinase 3
RNA