J Korean Ophthalmol Soc.  2001 Aug;42(8):1220-1226.

Secretion of Interleukin-8 from Human Keratocyte Stimulated by Aspergillus fumigatus and Effect of Amphotericin B and Dexamethasone on The Secretion

• Lee DJ
• Ahn GS
• Rho JH
• Hahn YH

Abstract

PURPOSE: To measure the secretion of IL-8 from cultured human keratocytes after inoculation of conidia of A. fumigatus, and to compare the change of secretion of IL-8 following application of amphotericin B and dexamethasone.
METHODS
Human corneal keraoctytes were cultured independently in vitro. The specimens were divided into 4 groups : Group I with only pure culture as control, Group II with conidia of A. fumigatus, Group III with conidia of A. fumigatus and amphotericin B, and Group IV with conidia of A. fumigatus and dexamethasone. The supernatants were aspirated from each group at different time intervals, and then were assayed for IL-8.
RESULTS
Group II showed increased secretion of IL-8, at all selected time intervals except 12-h, in comparison with other three groups. Group III secreted IL-8 significantly less than the other groups(p<0.01). Group IV secreted IL-8 less than Group I and II at all selected time points(p<0.01), but more than Group III at the other time points except for 72-h(p<0.01).
CONCLUSIONS
The secretion of IL-8 increased in the early stage of fungal keratitis but decreased in case of the administration of amphotericin B or dexamethasone. Amphotericin B was more potent than dexamethasone in decreasing the secretion of IL-8.

Keyword

Amphotericin B; Aspergillus fumigatus; Dexamethasone; IL-8; Keratocytes

MeSH Terms

Amphotericin B*
Aspergillus fumigatus*
Aspergillus*
Dexamethasone*
Humans*
Interleukin-8*
Keratitis
Spores, Fungal
Amphotericin B
Dexamethasone
Interleukin-8