J Korean Ophthalmol Soc.  1996 May;37(5):776-781.

A Comparative Evaluation of 4 degrees C Corneal Preservation Media, Optisol(R) and Likorol(R)

• Kim IS
• Kim MS

Abstract

We examined two different corneal preservatives, Optisol(R) and Likorol(R) which contain different concentrations of chondroitin sulfate and evaluated their ability to maintain cellular viability and corneal deturgescence. The corneal thickness(12 days storage, at 4 degrees C) and endothelial cell cytotoxicity (24, 48, 72, and 120 hours incubation at 35.5 degrees C) were measured in 8 paired human corneas. Corneas stored in Optisol were thinner than Likorol-stored corneas throughout the examination period. Alkaline Phosphatase activity and (3H)-Thymidine incorporation showed increased mitotic activity in endothelial cells cultured in Optisol when compared with Likorol-cultured cells. These results suggest that Optisol provides better maintenance of corneal deturgescence and increased corneal endothelial viability with prolonged storage time.

Keyword

Chondroitin sulfate containing media; Extended 4 degrees C Corneal storage; Likorol; Optisol

MeSH Terms

Alkaline Phosphatase
Chondroitin Sulfates
Cornea
Endothelial Cells
Humans
Alkaline Phosphatase
Chondroitin Sulfates