J Korean Soc Plast Reconstr Surg.
2000 May;27(3):240-246.
Expression of Proliferatin Cell Nuclear Antigen ( PCNA ) and P53 Protein in Skin Carcinomas
- Affiliations
-
- 1Department of Plastic and Reconstructive Surgery, Chonnam University Medical School, Kwangju, Korea.
Abstract
- Basal cell carcinoma(BCC) and squamous cell carcinoma(SCC) are the most frequent
neoplasms of the human skin. Ultraviolet radiation in sunlight is a well established mutagen
of p53 gene and is the major epidemiological risk factor in the development of basal cell
carcinoma and squamous cell carcinoma. There is no general agreement in the several studies
about the percentage of p53 positive staining of BCCs and SCCs. To evaluate the relationship
between p53 positivity, PCNA index and other clinicopathological parameters,
immunohistochemistry for p53 protein and PCNA was performed in 20 cases of BCCs, 14 SCCs
and 3 Bowen's disease. The results are as follows; 1) The immunostaining of p53 protein and
PCNA is localized to the nuclei of the tumor cells and some of adjacent normal surface squamous
epithelium, more intense in the periphery of the tumor nests. 2) The pattern of immunostaining
of PCNA is similar to that of p53 protein. 3) There was no difference in the expression of
p53 protein according to the location of tumors, age or sex of patients, except thickness.
4) There was no difference among the BCCs, SCCs and Bowen's disease in the percentage of
positive staining of p53 protein and PCNA but the aggressive BCCs and the less differentiated
SCCs showed more high PCNA index and more intense p53 immunostaining. Above results suggest
that the expressions of p53 protein and PCNA are related to the differentiation and biological
behavior such as infiltrating property of the tumor, and they may be used as adjuvant
indicators of prognosis in the skin carcinomas.