Secondary behavior changes of fibroblast functions induced by conditioned medium of silicone-sensitized murine lymphocytes

Rhie, Jong Won; Choi, Hang Seok; Byeon, Jun Hee; Won, Young Taik; Lee, Chong Kun; Han, Sang Bae; Kim, Hwan Mook

Abstract

Fibrous capsular contracture has been considered as a major side effect of breast silicone implant. The etiology of fibrous capsular contracture has not been fully determined. In the present study, we tried to determine the indirect effect of immune system in fibroblast function which plays a major role in fibrous capsular contracture. For preparation of conditional medium of lymphocytes, mouse (ICR) splenocytes were cultured for one day. Two kinds of conditioned medium, silicone conditioned medium (SCM) and silicone free normal conditioned medium (NCM), were prepared from splenocyte cell suspension cultured on silicone gel coated surface and naked surface, respectively. Mouse fibroblasts (NIH 3T3) were cultured in usual culture dish. Conditioned medium of 25% concentration was added. On day 2 after innoculation, cell number, thymidine incorporation and proline uptake of fibroblasts were measured. The results were as follow; 1) There was no difference of fibroblast number by cultivation in SCM of splenocytes compared with that in fresh medium(FM). 2)There was significant increase of DNA synthesis of fibroblasts in SCM compared with that in FM (p < 0.001). 3) There was significant increase of collagen synthesis of fibroblasts in SCM compared with that in FM (p < 0.01) and in NCM (p <0.001). 4) The functional activities of DNA and collagen synthesis mediated by same number of fibroblast were calculated to be significantly increased in SCM compared with that in NCM and in FM (p < 0.001). In conclusion, fibroblasts cultured in SCM had a higher potential to synthesize macromolecules such as collagen and DNA. We can postulate that SCM may contain certain amount of unknown growth stimulant of fibroblasts, which is produced by silicone gel sensitized murine splenocytes.