J Korean Ophthalmol Soc.  1992 Apr;33(4):381-387.

Effect of Conditioned Medium of Cultured Retinal Pigment Epithelium on Proliferation of Vascular Endothelial Cells in Bovine; In Vitro Study

• Kim JH
• Lee YC
• Hahn TW
• Huh W

Abstract

Neovascularization is a serious complication and major cause of visual loss in ocular diseases such as diabetic retinopathy and proliferative vitreoretionpathy. So there have been many attempts to inhibit neovascularization. Most inhibitors of neovascularzation so far identified have been extracted from lens and vitreous, and recently retinal pigment epitheliumin in culture also secrete angiogenic inhibitors. We cultured retinal pigment epithelial cell, vascular endothelial cell of Umblical vein and subconjunctival fibroblast of bovine. We studied the inhibitory effect in each of retinal pigment epithelium conditioned medium (RPE-CM) and fibroblast-CM on proliferation and migration of vascular endothelial cell comparing the effect of normal media group. In RPE-CM, proliferation and migration of vascular endotrelial cells were inhibited and the inhibition was dose-dependant. But in fibroblast-CM, we didn't observe the inhibitory effect These results suggest that in RPE-CM, there were certam substances to inhibit vascular endothelial cell proliferation and migration. Further research should be continued to detect that substance in molecular biological level.

Keyword

Neovascularization; RPE cell; RPE-CM; Fibroblast-CM proliferation; Migration

MeSH Terms

Angiogenesis Inhibitors
Culture Media, Conditioned*
Diabetic Retinopathy
Endothelial Cells*
Epithelial Cells
Fibroblasts
Retinal Pigment Epithelium*
Retinaldehyde*
Veins
Angiogenesis Inhibitors
Culture Media, Conditioned
Retinaldehyde