Korean J Blood Transfus.
2012 Aug;23(2):173-179.
Two Cases of Anti-Jka Whose Reactivity Was Disappeared after Enzyme Treatment
- Affiliations
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- 1Department of Laboratory Medicine, Chonbuk National University Medical School, Jeonju, Korea. choyg@jbnu.ac.kr
- 2Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Korea.
- 3Chonbuk National University Hospital Culture Collection for Pathogens, Jeonju, Korea.
Abstract
- We report on two cases of anti-Jka, whose reactivity disappeared on an antibody identification test using enzyme-treated red cells. One of two patients was a 72-year-old female with cirrhosis of the liver and colon cancer, and the other was a 55-year-old female with known MDS and incomplete Behcet's disease. Results of an antibody identification test using a LISS/Coombs gel card (DiaMed AG) showed negative to one positive with red cells having the Jka antigen; however, all reactions using the enzyme-treated cells showed negative results, which was unexpected. The patients' RBC phenotype was Jk(a-b+). We obtained positive results in reactions of enzyme-treated Jka+ cells and EDTA using a patient's serum and proved that the cause of the negative reaction might be complement-related.
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