Lab Anim Res.  2010 Dec;26(4):377-384. 10.5625/lar.2010.26.4.377.

The Symptoms of Atopic Dermatitis in NC/Nga Mice Were Significantly Relieved by the Water Extract of Liriope platyphylla

• Kim JE
• Lee YK
• Nam SH
• Choi SI
• Goo JS
• Jang MJ
• Lee HS
• Son HJ
• Lee CY
• Hwang DY

• KMID: 2114706
• DOI: http://doi.org/10.5625/lar.2010.26.4.377

Abstract

Atopic dermatitis is an well-known skin disease showing inflammatory, chronically relapsing, non-contagious and pruritic symptoms. The aims of this study were to investigate the effects of Liriope platyphylla (LP) on atopic dermatitis of NC/Nga mice. To achieve this, NC/Nga mice were treated with four different conditions including vehicle, phthalic anhydride (PA), PA+5% LP and PA+10% LP, and the changes of immune-related factors were detected after 2 weeks. The pathological phenotypes of atopic dermatitis such as erythema, ear thickness, edema, scab and discharge were significantly decreased in PA+10% LP cotreated groups compare to PA treated group. Also, the weight of lymph node and thymus in immune organs were gradually decreased in LP treated groups, while the weight of spleen was slightly increased in same group. Furthermore, toluidine blue staining analysis, a method used to specifically identify the mast cell, showed that the decrease of master cell infiltration into the dermis were statistically observed in PA+5% LP and PA+10% LP5% cotreated groups. Especially, the decrease of IgE concentration was detected only PA+10% LP cotreated group, although this level was maintained in PA+5% LP cotreated group. Therefore, these results suggested that the water extracts of LP may contribute the relieve of atopic dermatitis symptoms and be considered as an excellent candidate for a atopic dermatitis-therapeutic drug.

Keyword

Atopic dermatitis; Liriope platyphylla; mast cell; IgE; ear thickness

MeSH Terms

Animals
Dermatitis, Atopic
Dermis
Ear
Edema
Erythema
Immunoglobulin E
Lymph Nodes
Mast Cells
Mice
Phenotype
Phthalic Anhydrides
Skin Diseases
Spleen
Thymus Gland
Tolonium Chloride
Water
Immunoglobulin E
Phthalic Anhydrides
Tolonium Chloride
Water

Figure

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  Figure 1. Effects of Liriope platyphylla (LP) water extracts on the ear pathological phenotypes, the body weight and the ear thickness. Phthalic anhydride (PA) solution was repeatedly applied to the dorsum of the ear and back skin of NC/Nga mice. After 2 weeks, the difference in the skin irritation between PA treated group and PA+LP cotreated group was determined based on the change of ear pathological phenotype (A), the body weight (B) and the ear thickness (C) using the procedure described in the Materials and Methods. Data shown are the means±SD (n=5). ∗P<0.05 is the significance level compared to the vehicle treated group. ∗∗P<0.05 is the significance level compared to the PA treated group.

• 

  Figure 2. Effects of Liriope platyphylla (LP) water extracts on weight of three immune organs. At fourteenth days after LP extracts treatment, all of the animals were immediately sacrificed using CO2 gas in order to prepare the immune organs. Lymph nodes, spleens and thymus were collected from animals of all groups. And then, their weight were measured using the chemical balances. Data shown are the means±SD (n=5). ∗P<0.05 is the significance level compared to the vehicle treated group. ∗∗P<0.05 is the significance level compared to the phthalic anhydride (PA) treated group.

• 

  Figure 3. Effects of Liriope platyphylla (LP) water extracts on the pathological change of ear. The slide sections of ear tissue were stained with hematoxylin & eosin and observed at the original magnification ×40 or ×200. In addition, the arrows indicated the width of ear skin including epidermis, dermis and subcutaneous layer.

• 

  Figure 4. Effects of Liriope platyphylla water extracts on the mast cell infiltration. (A) The slide sections of ear tissue were stained with toluidine blue and observed at 400x magnification. Mast cells were stained with purple color in the dermis of ear tissue. (B) In each slide, five fields were randomly chosen and the number of mast cells was counted under a light microscope. The values are mean±SD. ∗P<0.05 is the significance level compared to the vehicle treated group. ∗∗P<0.05 is the significance level compared to the phthalic anhydride (PA) treated group.

• 

  Figure 5. Effects of Liriope platyphylla (LP) water extracts on the serum IgE concentration. After PA and different concentration of LP cotreatment, the mice were sacrificed under anesthesia. The serum was prepared from blood samples collected from the abdominal vein of the mice. The IgE concentration in serum was quantified by an enzyme-linked immunosorbent assay. Data shown are the means±SD (n=5). ∗P<0.05 is the significance level compared to the vehicle treated group. ∗∗P<0.05 is the significance level compared to the phthalic anhydride (PA) treated group.

Reference

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