J Korean Ophthalmol Soc.
1998 Aug;39(8):1801-1807.
Collagen Synthesis by RPE Cells Associated with Proliferative Vitreoretinopathy
- Affiliations
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- 1Institute of Vision Research, Yonsei University College of Medicine, Seoul, Korea.
- 2Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea.
Abstract
- Collagen, which is newly synthesized by the cellular component, is the major component of extracellular matrix in PVR membranes. The effects of transforming growth factor (TGF)-beta in the synthesis of collagen were evaluated. Tissue culture of PVR membrane was incubated for 19 hours with or without TGF-beta in the absence of serum. Newly synthesized collagen in the membrane were measured by a collagenase-digestion assay. The biosynthetically labeled collagens were analyzed on SDS-polyacrylamide gel electrophoresis. Collagen synthesis was dose-dependently stimulated by TGFbeta. At a concentration of 1ng/ml of TGFbeta1or TGFbeta2,at a concentration of 10ng/ml, induced a 90125% increase in collagen synthesis over that of controls. PVR membranes in culture synthesized predominantly type I, III and V collagen. In conclusion TGFbeta are potent stimulants of collagen.
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