J Korean Orthop Res Soc.
2003 Oct;6(2):259-264.
Factor V Leiden Gene Mutation in Femoral Head Osteonecrosis
- Affiliations
-
- 1Department of Orthopedic Surgery, School of Medicicne, Kyungpook National University. syukim@knu.ac.kr
- 2Department of clinical Pathology, School of Medicicne, Kyungpook National University.
- 3Skeletal Genome Research Center, Biomedical Research Institute, Kyungpook National University Hospital.
- 4Department of Dental Microbiology, School of Dental Medicine, Kyungpook National University.
- 5Department of Orthopedic Surgery, School of Medicicne, Seoul National University Hospital.
- 6Department of Preventive Medicine, School of Medicicne, Yeungnam University.
Abstract
- PURPOSE
The purpose of the present study was to evaluate the potential association of the G1691A mutation of factor V (factor V Leiden), which is a main causative factor of activated protein C resistance leading to intravascular coagulation, with osteonecrosis (ON) of the femoral head.
MATERIALS AND METHODS
Genomic DNA was extracted from peripheral blood leukocytes of 116 consecutively identified patients with nontraumatic ON of the femoral head and 59 healthy controls. The region in exon 10, that encodes an APC cleavage site in factor V gene, was amplified by polymerase chain reaction (PCR) with use of the 2 primers (Korea Biotech Inc., Daejeon): 5'-GGA ACA ACA CCA TGA TCA GAG CA-3' (forward primer) and 5'-TAG CCA GGA GAC CTA ACA TGT TC-3'(reverse primer). Amplified product was subjected to MnlI restriction enzyme digestion and resulting fragments were separated by electrophoresis on 3% agarose gel. The homozygous and heterozygous patterns of DNA fragments of 1691G-A mutation in the factor V gene was investigated.
RESULTS
The prevalence of factor V Leiden was 0% in the patients group and in the control group.
CONCLUSIONS
The data suggested that thrombophilia by the G1691A mutation of factor V (factor V Leiden) was less likely to be associated with the development of ON of the femoral head in Koreans.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- The Early diagnostic Significance of Bone Marrow Pressure in Osteonecrosis of the Femoral Head
- Study on the Factor V Leiden Mutation in Pregnant Women and Relationship with Preeclampsia Severe Form
- Posttraumatic Osteonecrosis of the Femoral Head after Nine Years of Posterior Femoral Head Fracture Dislocation
- Traumatic and Non-traumatic Osteonecrosis in the Femoral Head of a Rabbit Model
- Role of Mesenchymal Stem Cells in Patients with Nontraumatic Osteonecrosis of the Femoral Head
