J Korean Pain Soc.  2004 Jun;17(1):1-4. 10.3344/jkps.2004.17.1.1.

Microscopic Findings in a Muscular Pain Induced Murine Model

• Ha DH
• Cho YW
• Choi YS
• Lee KC
• Lee JH

• KMID: 2104816
• DOI: http://doi.org/10.3344/jkps.2004.17.1.1

Abstract

BACKGROUND
It has been suggested that muscular pain may induce skeletal muscle damage. But the mechanism of such skeletal muscle damage is not known. This study was performed to investigate the effect of carrageenan induced muscular pain on the tibialis anterior muscle of the rat. METHODS: Muscular pain was induced by injecting 0.1 ml of carrageenan into the tibialis anterior muscle of Sprague-Dawley rats. 48 hours after carrageenan injection, animals were killed, and tissues were fixed in 10% neutral buffered formalin (NBF). The tissues were then dehydrated, embedded, sectioned at 5mum, stained with phosphotungstic acid hematoxylin (PTAH), and observed under a light microscope. RESULTS: Carrageenan induced muscular pain was associated with a wave-like arrangement of muscle cells, which were separated by intercellular gaps in tibialis anterior muscle. CONCLUSIONS: This finding suggests that carrageenan may induce muscular pain damage in the tibialis anterior muscle of rat, as evidenced by a divided wave like muscle cell arrangement.

Keyword

carrageenan; pain; skeletal muscle; tibialis anterior muscle

MeSH Terms

Animals
Carrageenan
Formaldehyde
Hematoxylin
Muscle Cells
Muscle, Skeletal
Phosphotungstic Acid
Rats
Rats, Sprague-Dawley
Carrageenan
Formaldehyde
Hematoxylin
Phosphotungstic Acid